[人胃肠道癌组织中的支原体感染]

[Mycoplasma infection in human gastriointestinal carcinoma tissues].

作者信息

Huang S, Shou C, Wu J

机构信息

BeiJing Institute for Cancer Research, Peking University School of Oncology, 100034, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2001 May 25;81(10):601-4.

PMID:11798932

Abstract

OBJECTIVE

To explore the association between the carcinoma and mycoplasma infection by immunohistochemistry.

METHODS

Antigen of anti-tumor monoclonal antibody PD4 was identified as mycoplasma hyorhinis. Using immunohistochemistry to study archived paraffin-embedded gastriointestinal tract carcinomas tissue. The PCR was applied to amplify the mycoplasma DNA for confirming immunohistochemistry results.

RESULTS

It was showed that mycoplasma was present in 56% (50/90 cases) of gastric carcinoma. The cancer tissues with high differentiation had a higher mycoplasma infection ratio than that of low differentiation cancer tissues. (P < 0.05). In control cases, mycoplasma infection was 28% (18/49) in chronic superficial gastritis, 30% (14/46) in gastric ulcer and 37% (18/49) in intestinal metaplasia of the stomach. Mycoplasma infection was 55.1% (32/58) in colon carcinoma and 20.9% (10/49) in adenomarous polyp (P < 0.005). It seems that colon carcinoma tissues with slight pathological grade had a higher percent of mycoplasma infection than that of cancer tissues with moderate and heavy pathological grade (P < 0.05).

CONCLUSION

The high infection of mycoplasmas in carcinoma tissues suggest an association between mycoplasma and cancer. The mechanism involved in oncogenesis by mycoplasmas remains to be elucidated.

摘要

目的

通过免疫组织化学方法探讨癌与支原体感染之间的关联。

方法

将抗肿瘤单克隆抗体PD4的抗原鉴定为猪鼻支原体。采用免疫组织化学方法研究存档的石蜡包埋胃肠道癌组织。应用聚合酶链反应（PCR）扩增支原体DNA以证实免疫组织化学结果。

结果

结果显示，56%（50/90例）的胃癌组织中存在支原体。高分化癌组织的支原体感染率高于低分化癌组织（P<0.05）。在对照病例中，慢性浅表性胃炎的支原体感染率为28%（18/49），胃溃疡为30%（14/46），胃肠化生为37%（18/49）。结肠癌的支原体感染率为55.1%（32/58），腺瘤性息肉为20.9%（10/49）（P<0.005）。似乎病理分级较轻的结肠癌组织的支原体感染率高于病理分级中度和重度的癌组织（P<0.05）。