Zhang H, Lin C, Shao Y
Department of Abdominal Surgery, Cancer Hospital, National Laboratory of Molecular Oncology, Chinese Academy of Medical Science, Peking Union Medical College, Beijing, 100021 China.
Zhonghua Yi Xue Za Zhi. 2001 Jun 10;81(11):673-6.
To investigate the therapeutic effects of Adenovirus-transferred antisense c-myc (Ad-Asmyc) on hepatocellular cancer cells and its mechanism.
Human hepatocellular cancer cell line BEL-7402 and QSG-7701 were transfected with Ad-Asmyc. Cell growth curve and coloning formation were used to investigate the therapeutic effects of Ad-Asmyc in Vitro. The treating effects of Ad-Asmyc on transplanted tumor in nude mice was also studied. RT-PCR and Western blot were used to detect relating gene expression. Flowcytometry, DNA ladder and tunnel assay were used to study the cell cycle and apoptosis.
The expression of c-myc in cancer cells was reduced after infected Ad-Asmyc. Ad-Asmyc had obviously inhibiting effects on the growth of two hepatocellular carcinoma cell lines in vitro and also in vivo. It can induced cell cycle arrest (G1 arrest) and apoptosis.
Ad-Asmyc can inhibit HCC cell proliferation and induce apoptosis, so has obvious therapeutic effects on hepatocellular cancer cells.
探讨腺病毒介导的反义c-myc（Ad-Asmyc）对肝癌细胞的治疗作用及其机制。
用Ad-Asmyc转染人肝癌细胞系BEL-7402和QSG-7701。采用细胞生长曲线和克隆形成实验观察Ad-Asmyc的体外治疗作用。研究Ad-Asmyc对裸鼠移植瘤的治疗作用。采用RT-PCR和Western blot检测相关基因表达。运用流式细胞术、DNA梯状条带分析和隧道试验研究细胞周期和凋亡情况。
感染Ad-Asmyc后癌细胞中c-myc表达降低。Ad-Asmyc对两种肝癌细胞系的体外和体内生长均有明显抑制作用。它可诱导细胞周期阻滞（G1期阻滞）和凋亡。
Ad-Asmyc能抑制肝癌细胞增殖并诱导凋亡，对肝癌细胞有明显治疗作用。
