Li Z, Qu J, He L
Department of Pulmonary Medicine, Zhongshan Hospital, Institute of Respiratory Diseases, Medical Center of Fudan University, Shanghai 200032, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2001 Apr;24(4):204-7.
To analyze the alterations of pulmonary surfactant in immunocompromised host with Pseudomonas aeruginosa (PA) pneumonia.
50 rats were randomly divided into two groups, one immunosuppressed with cyclophosphamide and cortisone acetate as ICH group, another as control group (CON), their lung tissue and bronchoalveolar lavage fluid (BALF) were collected before PA challenging and 3 h, 6 h, 9 h, 24 h after PA challenging intratracheally, wet/dry ratio (W/D) of lung tissue were measured, concentrations of total protein (TP), total phospholipids (TPL), disaturated phosphatidylcholine (DSPC) and surfactant protein A (SP-A) in BALF were analyzed.
The ratios of DSPC/TPL and DSPC/TP decreased significantly in both ICH and CON groups after PA infection, there were not significant differences between the two groups, no change in the concentrations of TPL and DSPC. Concentration of SP-A and SP-A/TP in ICH group decreased remarkably 6 h after PA pulmonary infection than before [(1.8 +/- 1.1) microgram/ml vs (4.2 +/- 1.5) microgram/ml, (1.4 +/- 0.7) microgram/mg vs (11.7 +/- 8.1) microgram/mg, P < 0.05], meanwhile there were no significant alterations in CON group. W/D and TP concentrations increased in both groups after PA challenging, however the alterations were much greater in ICH group (P < 0.05). Alterations of SP-A appeared a negative correlation with alterations of TP and W/D (r = -0.793, P < 0.01, r = -0.769, P < 0.01). The ratios of SP-A/TPL and SP-A/DSPC decreased significantly 6 h after PA challenging than before, the ratios were much lower in ICH group than in CON group during 6 h-9 h after PA inoculation.
After PA pulmonary infection, alterations of phospholipids could initially appear a relative decrement of DSPC during acute phase of infection, much remarkable decrease of SP-A in ICH group could be associated with the more severe lung injury, alterations of SP-A were more obvious than surfactant lipids in ICH.
分析免疫功能低下宿主合并铜绿假单胞菌（PA）肺炎时肺表面活性物质的变化。
将50只大鼠随机分为两组，一组用环磷酰胺和醋酸可的松免疫抑制作为免疫功能低下组（ICH组），另一组作为对照组（CON组），在气管内接种PA前及接种后3小时、6小时、9小时、24小时收集其肺组织和支气管肺泡灌洗液（BALF），测定肺组织湿/干比（W/D），分析BALF中总蛋白（TP）、总磷脂（TPL）、二饱和磷脂酰胆碱（DSPC）和表面活性蛋白A（SP-A）的浓度。
PA感染后，ICH组和CON组的DSPC/TPL及DSPC/TP比值均显著降低，两组间无显著差异，TPL和DSPC浓度无变化。PA肺部感染6小时后，ICH组SP-A浓度及SP-A/TP显著低于感染前[（1.8±1.1）μg/ml对（4.2±1.5）μg/ml，（1.4±0.7）μg/mg对（11.7±8.1）μg/mg，P<0.05]，而CON组无显著变化。PA攻击后两组W/D和TP浓度均升高，但ICH组变化更大（P<0.05）。SP-A变化与TP和W/D变化呈负相关（r=-0.793，P<0.01，r=-0.769，P<0.01）。PA攻击6小时后SP-A/TPL和SP-A/DSPC比值显著低于攻击前，PA接种后6小时至9小时，ICH组比值显著低于CON组。
PA肺部感染后，感染急性期磷脂变化最初表现为DSPC相对减少，ICH组SP-A显著降低可能与更严重的肺损伤有关，ICH组SP-A变化比表面活性脂质更明显。
