Böhmer J A, Sellhaus B, Schrage N F
Interdisciplinary Centre of Clinical Research (IZKF) BIOMAT, Technical University of Aachen, Germany.
Curr Eye Res. 2001 Sep;23(3):206-14. doi: 10.1076/ceyr.23.3.206.5464.
Evaluation of effects of ascorbic acid on cell characteristics of dedifferentiated porcine retinal pigment epithelial (pRPE) cells.
pRPE cells were incubated in vitro with increasing concentrations of ascorbic acid (0.25-1.5 mMol). Cell proliferation was assayed by measuring the incorporation of 5-bromo-2'-deoxy-uridine (BrdU) into cellular DNA. Migration and contraction properties were studied on a cell permissive porous membrane and collagen gels, respectively. Phenotypic changes in response to ascorbic acid and its derivative ascorbic acid 2-phophate were evaluated by microscopy and indirect immunofluorescence.
Ascorbic acid significantly inhibits cell proliferation, migration, and contraction in concentrations of 1 mMol or more. Under the influence of at least 1 mMol ascorbic acid dedifferentiated pRPE cells exhibited a pigmented status within 24 hours. Addition of 500 U/ml catalase prevented the antiproliferative effect of ascorbic acid and the formation of pigment. Concentrations of 0.5 mMol ascorbic acid as well as 1 mMol ascorbic acid 2-phosphate promoted differentiation of cell phenotype. Furthermore, ascorbic acid 2-phosphate supported the formation of in vivo-like epithelial structures.
Ascorbic acid has an influence on vital cell characteristics such as proliferation, migration, contraction and differentiation of pRPE cells. As dedifferentiation of these cells is an integral part in the development of proliferative vitreoretinopathy (PVR), ascorbic acid should be taken into consideration as a supplement in the clinical management of this disease.
评估抗坏血酸对去分化猪视网膜色素上皮（pRPE）细胞的细胞特性的影响。
将pRPE细胞在体外与浓度不断增加的抗坏血酸（0.25 - 1.5毫摩尔）一起孵育。通过测量5-溴-2'-脱氧尿苷（BrdU）掺入细胞DNA来检测细胞增殖。分别在允许细胞通过的多孔膜和胶原凝胶上研究迁移和收缩特性。通过显微镜检查和间接免疫荧光评估对抗坏血酸及其衍生物抗坏血酸2-磷酸的表型变化。
抗坏血酸在浓度为1毫摩尔或更高时显著抑制细胞增殖、迁移和收缩。在至少1毫摩尔抗坏血酸的影响下，去分化的pRPE细胞在24小时内呈现色素沉着状态。添加500 U/ml过氧化氢酶可防止抗坏血酸的抗增殖作用和色素形成。0.5毫摩尔抗坏血酸以及1毫摩尔抗坏血酸2-磷酸的浓度促进细胞表型分化。此外，抗坏血酸2-磷酸支持体内样上皮结构的形成。
抗坏血酸对pRPE细胞的增殖、迁移、收缩和分化等重要细胞特性有影响。由于这些细胞的去分化是增殖性玻璃体视网膜病变（PVR）发展的一个组成部分，抗坏血酸应作为这种疾病临床治疗中的一种补充剂加以考虑。
