Yaneza May, Gilthorpe Jonathan D, Lumsden Andrew, Tucker Abigail S
MRC Centre for Developmental Neurobiology, King's College London, Guy's Hospital, London, United Kingdom.
Dev Dyn. 2002 Jan;223(1):163-7. doi: 10.1002/dvdy.1241.
Abstract The neural crest is a migratory population of cells that originates from the dorsal neural tube in vertebrates. Recently, the existence of a group of ventrally emigrating neural tube (VENT) cells has been proposed, based upon cell labelling studies in the hindbrain of avian embryos. Like crest cells, these VENT cells have been reported to give rise to numerous cell types. VENT cell emigration is thought to occur after embryonic day (E) 3, when neural crest cell production has ceased. Migration of cells from the ventral neural tube into the periphery was inferred retrospectively after examining numerous embryos harvested at different stages. We have attempted to label VENT cells in vivo by using a green fluorescent protein (GFP) expression vector, electroporated into the ventral neural tube after crest cell migration and before the putative migration of the ventrally localised cells. Because GFP can be visualised strongly in living tissue a few hours after electroporation, the migration of labelled cells within the same embryo can be followed. Fluorescent cells labelled in the mid-hindbrain region were examined in ovo and in explant culture. No GFP-expressing cells were detected emigrating from the ventral neural tube from E3 to E5. Our findings are, thus, in disagreement with those of previous studies, which have indicated the existence of VENT cells in the cranial region.
摘要 神经嵴是脊椎动物中起源于背侧神经管的一群迁移细胞。最近，基于对鸡胚后脑的细胞标记研究，有人提出存在一群从腹侧迁出的神经管（VENT）细胞。与嵴细胞一样，据报道这些VENT细胞可分化为多种细胞类型。VENT细胞的迁出被认为发生在胚胎期（E）3之后，此时神经嵴细胞的产生已经停止。在检查了不同阶段收获的大量胚胎后，回顾性推断细胞从腹侧神经管向周边的迁移情况。我们试图通过使用绿色荧光蛋白（GFP）表达载体在体内标记VENT细胞，该载体在嵴细胞迁移后且在假定的腹侧定位细胞迁移之前电穿孔导入腹侧神经管。由于电穿孔后数小时即可在活体组织中强烈观察到GFP，因此可以追踪同一胚胎内标记细胞的迁移。对中后脑区域标记的荧光细胞进行了胚胎内和外植体培养观察。在E3至E5期间，未检测到从腹侧神经管迁出的表达GFP的细胞。因此，我们的研究结果与先前表明颅区存在VENT细胞的研究结果不一致。
