O(2)-钴（II）博来霉素-小牛胸腺DNA加合物的拉曼光谱：交替的聚合物构象

Raman spectroscopy of an O(2)-Co(II)bleomycin-calf thymus DNA adduct: alternate polymer conformations.

作者信息

Rajani C, Kincaid J R, Petering D H

机构信息

Department of Chemistry, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53211, USA.

出版信息

Biophys Chem. 2001 Dec 25;94(3):219-36. doi: 10.1016/s0301-4622(01)00237-x.

DOI:10.1016/s0301-4622(01)00237-x

PMID:11804732

Abstract

Bleomycin (Blm) is an antitumor agent which binds to specific sequences of DNA and as HO(2)-Fe(III)Blm causes single and double strand cleavage. In the present investigation, binding of O(2)-Co(II)Blm to a native DNA polymer, calf thymus DNA, was examined using conventional Raman spectroscopy. O(2)-Co(II)Blm is a model for O(2)-Fe(II)Blm, the direct precursor of HO(2)-Fe(III)Blm. Although the DNA polymer retained a predominant B-form structure, Raman spectral evidence was obtained for localized structural changes to A, C and Z-DNA forms. The presence of these alternate DNA forms within B-DNA implied the presence of B/A, B/C and B/Z junctions. The observed changes in DNA secondary structure were attributed to perturbation of structural water resulting from binding of O(2)-Co(II)Blm within the minor groove.

摘要

博来霉素（Blm）是一种抗肿瘤药物，它能与DNA的特定序列结合，并且作为HO(2)-Fe(III)Blm会导致单链和双链断裂。在本研究中，使用传统拉曼光谱法检测了O(2)-Co(II)Blm与天然DNA聚合物小牛胸腺DNA的结合情况。O(2)-Co(II)Blm是O(2)-Fe(II)Blm的模型，而O(2)-Fe(II)Blm是HO(2)-Fe(III)Blm的直接前体。尽管DNA聚合物保留了主要的B型结构，但获得的拉曼光谱证据表明存在向A、C和Z-DNA形式的局部结构变化。这些B-DNA内交替DNA形式的存在意味着存在B/A、B/C和B/Z连接。观察到的DNA二级结构变化归因于O(2)-Co(II)Blm在小沟内结合导致的结构水扰动。