School of Public Health, Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Biomolecules. 2024 Sep 19;14(9):1173. doi: 10.3390/biom14091173.
DNA adductomics is the global study of all DNA adducts and was first proposed in 2006 by the Matsuda group. Its development has been greatly credited to the advances in mass spectrometric techniques, particularly tandem and multiple-stage mass spectrometry. In fact, liquid chromatography-mass spectrometry (LC-MS)-based methods are virtually the sole technique with practicality for DNA adductomic studies to date. At present, DNA adductomics is primarily used as a tool to search for DNA adducts, known and unknown, providing evidence for exposure to exogenous genotoxins and/or for the molecular mechanisms of their genotoxicity. Some DNA adducts discovered in this way have the potential to predict cancer risks and/or to be associated with adverse health outcomes. DNA adductomics has been successfully used to identify and determine exogenous carcinogens that may contribute to the etiology of certain cancers, including bacterial genotoxins and an -nitrosamine. Also using the DNA adductomic approach, multiple DNA adducts have been observed to show age dependence and may serve as aging biomarkers. These achievements highlight the capability and power of DNA adductomics in the studies of medicine, biological science, and environmental science. Nonetheless, DNA adductomics is still in its infancy, and great advances are expected in the future.
DNA 加合物组学是对所有 DNA 加合物的全局研究，于 2006 年由松田小组首次提出。其发展主要归功于质谱技术的进步，特别是串联和多级质谱。事实上，基于液相色谱-质谱（LC-MS）的方法实际上是迄今为止用于 DNA 加合物组学研究的唯一实用技术。目前，DNA 加合物组学主要用作寻找已知和未知 DNA 加合物的工具，为外源性遗传毒物的暴露及其遗传毒性的分子机制提供证据。通过这种方式发现的一些 DNA 加合物具有预测癌症风险和/或与不良健康结果相关的潜力。DNA 加合物组学已成功用于鉴定和确定可能导致某些癌症病因的外源性致癌物，包括细菌遗传毒物和亚硝胺。同样使用 DNA 加合物组学方法，已经观察到多种 DNA 加合物具有年龄依赖性，并可能作为衰老生物标志物。这些成就突出了 DNA 加合物组学在医学、生物科学和环境科学研究中的能力和潜力。尽管如此，DNA 加合物组学仍处于起步阶段，未来有望取得重大进展。
