Krogan Nevan J, Dover Jim, Khorrami Shahram, Greenblatt Jack F, Schneider Jessica, Johnston Mark, Shilatifard Ali
Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104, USA.
J Biol Chem. 2002 Mar 29;277(13):10753-5. doi: 10.1074/jbc.C200023200. Epub 2002 Jan 22.
The trithorax (Trx) family of proteins is required for maintaining a specific pattern of gene expression in some organisms. Recently we reported the isolation and characterization of COMPASS, a multiprotein complex that includes the Trx-related protein Set1 of the yeast Saccharomyces cerevisiae. Here we report that COMPASS catalyzes methylation of the fourth lysine of histone H3 in vitro. Set1 and several other components of COMPASS are also required for histone H3 methylation in vivo and for transcriptional silencing of a gene located near a chromosome telomere.
在一些生物体中，维持特定的基因表达模式需要trithorax（Trx）蛋白家族。最近我们报道了COMPASS的分离和特性，COMPASS是一种多蛋白复合体，包含酿酒酵母中与Trx相关的蛋白Set1。在此我们报道，COMPASS在体外催化组蛋白H3第四位赖氨酸的甲基化。Set1和COMPASS的其他几个组分在体内对组蛋白H3甲基化以及位于染色体端粒附近的一个基因的转录沉默也是必需的。
