• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

重组前原伴刀豆球蛋白A（洋刀豆）稳定但溶解度低。

Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.

作者信息

Dincturk H B, Dunn A J, Jones D H

机构信息

Molecular Biology Research Group, School of Biological Sciences, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, UK.

出版信息

J Biosci. 2001 Dec;26(5):635-40. doi: 10.1007/BF02704761.

DOI:10.1007/BF02704761
PMID:11807293
Abstract

The cDNA for pre-pro-Concanavalin A (pre-pro-ConA) was cloned into the cytoplasmic expression vector pKK233-2 to give rise to pCONEXP2 which was used to express the lectin precursor. Pre-pro-ConA is stable and is not transposed and ligated to form the mature protein. No signal peptide removal is observed. The solubility of pre-pro-ConA could not be increased by guanidine hydrochloride denaturation/dilution treatment.

摘要

将伴刀豆球蛋白A前体（pre-pro-ConA）的cDNA克隆到细胞质表达载体pKK233-2中，构建出pCONEXP2，用于表达凝集素前体。pre-pro-ConA是稳定的，不会发生转位和连接以形成成熟蛋白。未观察到信号肽的去除。盐酸胍变性/稀释处理无法提高pre-pro-ConA的溶解度。

相似文献

1
Recombinant pre-pro-Concanavalin A (jack bean) is stable but of low solubility.重组前原伴刀豆球蛋白A（洋刀豆）稳定但溶解度低。
J Biosci. 2001 Dec;26(5):635-40. doi: 10.1007/BF02704761.
2
Stability and detection of recombinant pre-pro-concanavalin A after cytoplasmic expression in Escherichia coli.重组伴刀豆球蛋白A前原蛋白在大肠杆菌细胞质中表达后的稳定性及检测
FEBS Lett. 1992 Apr 27;301(3):315-8. doi: 10.1016/0014-5793(92)80265-i.
3
Non-glycosylated recombinant pro-concanavalin A is active without polypeptide cleavage.
EMBO J. 1992 Apr;11(4):1303-7. doi: 10.1002/j.1460-2075.1992.tb05174.x.
4
Structural and biochemical analyses of concanavalin A circular permutation by jack bean asparaginyl endopeptidase.用刀豆氨酸内肽酶对伴刀豆球蛋白进行结构和生化分析的环形排列。
Plant Cell. 2021 Aug 31;33(8):2794-2811. doi: 10.1093/plcell/koab130.
5
Characterization of the endoplasmic reticulum-associated precursor of concanavalin A. Partial amino acid sequence and lectin activity.伴刀豆球蛋白A内质网相关前体的表征。部分氨基酸序列和凝集素活性。
J Biol Chem. 1986 Aug 5;261(22):10021-4.
6
Crystallization and X-ray diffraction data for a new form of concanavalin A.
Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):1032-3. doi: 10.1107/s090744490200642x. Epub 2002 May 29.
7
Deglycosylation is necessary but not sufficient for activation of proconcanavalin A.去糖基化对于伴刀豆球蛋白A原的激活是必要的，但并不充分。
J Exp Bot. 2001 May;52(358):911-7. doi: 10.1093/jexbot/52.358.911.
8
Synthesis of soybean agglutinin in bacterial and mammalian cells.
Eur J Biochem. 1997 Nov 1;249(3):684-9. doi: 10.1111/j.1432-1033.1997.t01-3-00684.x.
9
Bowringia mildbraedii agglutinin: polypeptide composition, primary structure and homologies with other legume lectins.鲍氏豆凝集素：多肽组成、一级结构及与其他豆科植物凝集素的同源性
Biochim Biophys Acta. 1993 Sep 3;1202(1):38-46. doi: 10.1016/0167-4838(93)90060-5.
10
A jack bean protein similar to pea seed ferritin and unrelated to concanavalin A is the only iron storage protein in jack bean seeds, although concanavalin A can form ferritin-like iron cores in vitro.一种与豌豆种子铁蛋白相似且与伴刀豆球蛋白A无关的刀豆蛋白是刀豆种子中唯一的铁储存蛋白，尽管伴刀豆球蛋白A在体外可形成类似铁蛋白的铁核。
J Biol Chem. 1989 Jul 15;264(20):11550-3.

引用本文的文献

1
Computational insights into the circular permutation roles on ConA binding and structural stability.对伴刀豆球蛋白A结合及结构稳定性中环形排列作用的计算洞察。
Curr Res Struct Biol. 2024 Mar 23;7:100140. doi: 10.1016/j.crstbi.2024.100140. eCollection 2024.
2
A simple strategy for the creation of a recombinant lectin microarray.一种构建重组凝集素微阵列的简单策略。
Mol Biosyst. 2008 Jun;4(6):654-62. doi: 10.1039/b800725j. Epub 2008 Apr 14.

本文引用的文献

1
Concanavalin A is synthesized as a glycoprotein precursor.刀豆球蛋白 A 作为糖蛋白前体被合成。
Planta. 1985 Jul;165(1):23-9. doi: 10.1007/BF00392207.
2
In vitro splicing of concanavalin A is catalyzed by asparaginyl endopeptidase.伴刀豆球蛋白A的体外剪接受天冬酰胺内肽酶催化。
Nat Struct Biol. 1994 Aug;1(8):502-4. doi: 10.1038/nsb0894-502.
3
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.原核生物、真核生物和细胞器中蛋白质合成起始的比较。
Microbiol Rev. 1983 Mar;47(1):1-45. doi: 10.1128/mr.47.1.1-45.1983.
4
Polypeptide ligation occurs during post-translational modification of concanavalin A.多肽连接发生在伴刀豆球蛋白A的翻译后修饰过程中。
Nature. 1985;313(5997):64-7. doi: 10.1038/313064a0.
5
Posttranslational processing of concanavalin A precursors in jackbean cotyledons.刀豆球蛋白A前体在刀豆种子子叶中的翻译后加工
J Cell Biol. 1986 Apr;102(4):1284-97. doi: 10.1083/jcb.102.4.1284.
6
Expression of a wheat alpha-amylase gene in Escherichia coli: recognition of the translational initiation site and the signal peptide.小麦α-淀粉酶基因在大肠杆菌中的表达：翻译起始位点和信号肽的识别
Gene. 1986;45(1):11-8. doi: 10.1016/0378-1119(86)90126-5.
7
Production of pea lectin in Escherichia coli.豌豆凝集素在大肠杆菌中的产生。
J Biol Chem. 1986 May 15;261(14):6141-4.
8
cDNAs for canavalin and concanavalin A from Canavalia gladiata seeds. Nucleotide sequence of cDNA for canavalin and RNA blot analysis of canavalin and concanavalin A mRNAs in developing seeds.来自刀豆种子的伴刀豆球蛋白和伴刀豆凝集素A的互补DNA。伴刀豆球蛋白的互补DNA核苷酸序列以及发育种子中伴刀豆球蛋白和伴刀豆凝集素A信使核糖核酸的RNA印迹分析。
Eur J Biochem. 1988 Jan 4;170(3):515-20. doi: 10.1111/j.1432-1033.1988.tb13730.x.
9
"ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli.用于在大肠杆菌中调控克隆基因高水平表达的“ATG载体”。
Gene. 1985;40(2-3):183-90. doi: 10.1016/0378-1119(85)90041-1.
10
Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells.
FEBS Lett. 1990 Jan 15;260(1):127-30. doi: 10.1016/0014-5793(90)80083-u.