Yang Bin, Boussaid Abdel, Mansfield Shawn D, Gregg David J, Saddler John N
Faculty of Forestry, Forest Products Biotechnology, University of British Columbia, Vancouver, Canada.
Biotechnol Bioeng. 2002 Mar 20;77(6):678-84. doi: 10.1002/bit.10159.
The enzymatic digestibility of steam-exploded Douglas-fir wood chips (steam exploded at 195 degrees C, 4.5 min, and 4.5% (w/w) SO(2)) was significantly improved using an optimized alkaline peroxide treatment. Best hydrolysis yields were attained when the steam-exploded material was post-treated with 1% hydrogen peroxide at pH 11.5 and 80 degrees C for 45 min. This alkaline peroxide treatment was applied directly to the water-washed, steam-exploded material eliminating the need for independent alkali treatment with 0.4% NaOH, which has been traditionally used to post-treat wood samples to try to remove residual lignin. Approximately 90% of the lignin in the original wood was solubilized by this novel procedure, leaving a cellulose-rich residue that was completely hydrolyzed within 48 h, using an enzyme loading of 10 FPU/g cellulose. About 82% of the originally available polysaccharide components of the wood could be recovered. The 18% of the carbohydrate that was not recovered was lost primarily to sugar degradation during steam explosion.
采用优化的碱性过氧化氢处理方法，可显著提高蒸汽爆破处理的花旗松木片（在195℃、4.5分钟和4.5%（w/w）SO₂条件下进行蒸汽爆破）的酶解消化率。当蒸汽爆破后的物料在pH值为11.5、80℃的条件下用1%的过氧化氢进行后处理45分钟时，可获得最佳水解产率。这种碱性过氧化氢处理直接应用于水洗后的蒸汽爆破物料，无需使用传统上用于后处理木材样品以试图去除残留木质素的0.4%氢氧化钠进行单独的碱处理。通过这种新方法，原木材中约90%的木质素被溶解，留下富含纤维素的残渣，使用10 FPU/g纤维素的酶负载量，该残渣在48小时内可完全水解。木材中约82%的原始可用多糖成分可以被回收。未回收的18%的碳水化合物主要在蒸汽爆破过程中因糖降解而损失。
