Basolo Fulvio, Giannini Riccardo, Toniolo Antonio, Casalone Rosario, Nikiforova Marina, Pacini Furio, Elisei Rossella, Miccoli Paolo, Berti Piero, Faviana Pinuccia, Fiore Lisa, Monaco Carmen, Pierantoni Giovanna Maria, Fedele Monica, Nikiforov Yuri E, Santoro Massimo, Fusco Alfredo
Dipartimento di Oncologia, Università degli Studi di Pisa, Pisa, Italy.
Int J Cancer. 2002 Feb 10;97(5):608-14. doi: 10.1002/ijc.10116.
A novel human thyroid papillary carcinoma cell line (FB-2) has been established and characterized. FB-2 cells harbor the RET/PTC1 chimeric oncogene in which the RET kinase domain is fused to the H4 gene. FB-2 cells neither formed colonies in semisolid media nor induced tumors after heterotransplant into severe combined immunodeficient mice. However, HMGI(Y), HMGI-C and c-myc genes, which are associated to thyroid cell transformation, were abundantly expressed in FB-2 cells but not in normal thyroid cells. FB-2 cells only partially retained the differentiated thyroid phenotype. In fact, the PAX-8 gene, which codes for a transcriptional factor required for thyroid cell differentiation, was expressed, while thyroglobulin, TSH-receptor and thyroperoxidase genes were not. Moreover, FB-2 cells produced high levels of interleukin (IL)-6 and IL-8.
一种新的人甲状腺乳头状癌细胞系（FB - 2）已被建立并进行了特征描述。FB - 2细胞含有RET/PTC1嵌合致癌基因，其中RET激酶结构域与H4基因融合。FB - 2细胞在半固体培养基中既不形成集落，异种移植到严重联合免疫缺陷小鼠后也不诱导肿瘤形成。然而，与甲状腺细胞转化相关的HMGI(Y)、HMGI - C和c - myc基因在FB - 2细胞中大量表达，而在正常甲状腺细胞中不表达。FB - 2细胞仅部分保留了分化的甲状腺表型。事实上，编码甲状腺细胞分化所需转录因子的PAX - 8基因表达了，而甲状腺球蛋白、促甲状腺激素受体和甲状腺过氧化物酶基因则未表达。此外，FB - 2细胞产生高水平的白细胞介素（IL）- 6和IL - 8。
