Grisez-Duranton C, Dorchies Ph, Jourdane J, Durand P
Laboratoire de Parasitologie UMR 959, Physiopathologie des Maladies Infectieuses et Parasitaires des Ruminants, Ecole Nationale Vétérinaire, 23 Chemin des Capelles, 31076 Toulouse Cedex 03, France.
Vet Parasitol. 2002 Mar 1;104(2):167-73. doi: 10.1016/s0304-4017(01)00617-3.
A genetic analysis using RAPD markers was performed on 12 natural populations of Oestrus ovis (Linné, 1761). Three-hundred and six O. ovis larvae (first, second and third instars) were randomly recovered in nasal cavities of sheep and goats naturally infected in Algeria, Ethiopia, France, Mauritania, Rumania and Tunisia and were analysed by 56 RAPD fragments. The results showed a high diversity within all samples. A significant genetic divergence was showed by discriminant analyses among the 12 populations sampled (p<0.0001). Moreover, discriminant analyses showed significant differentiation (p<0.0001) between O. ovis larva populations of sheep and goats and also among samples collected in the same region.
利用随机扩增多态性DNA（RAPD）标记对12个羊狂蝇（Oestrus ovis，林奈，1761年）自然种群进行了遗传分析。从阿尔及利亚、埃塞俄比亚、法国、毛里塔尼亚、罗马尼亚和突尼斯自然感染的绵羊和山羊鼻腔中随机采集了306只羊狂蝇幼虫（一龄、二龄和三龄），并通过56个RAPD片段进行分析。结果显示所有样本内均具有高度多样性。对所采集的12个种群进行判别分析，结果显示存在显著的遗传分化（p<0.0001）。此外，判别分析表明，绵羊和山羊体内的羊狂蝇幼虫种群之间以及在同一地区采集的样本之间均存在显著差异（p<0.0001）。
