Kite Geoffrey C, Ireland Helen
Royal Botanic Gardens, Kew, Richmond, Surrey TW9 3AB, UK.
Phytochemistry. 2002 Jan;59(2):163-8. doi: 10.1016/s0031-9422(01)00447-2.
The non-protein amino acids of the legume genus Bocoa (Papilionoideae; Swartzieae) were surveyed by LC-MS and GC-MS using extracts of herbarium leaf fragments. Bocoa alterna (Benth.) R.S. Cowan, B. decipiens R.S. Cowan, B. limae R.S. Cowan, B. mollis (Benth.) R.S. Cowan and B. racemulosa (Huber) R.S. Cowan were found to contain 2,4-methanoproline, 2,4-methanoglutamic acid, cis-1-amino-3-hydroxymethyl-cyclobutane-1-carboxylic acid and delta-N-acetylornithine. The former three compounds have otherwise only been reported from Ateleia and Cyathostegia and, therefore, the results support the relationship with these genera found in recent phylogenetic analysis of DNA sequence data. In contrast, Bocoa viridiflora (Ducke) R.S. Cowan was found to contain trans-5-hydroxypipecolic acid and trans-4-cis-5-dihydroxypipecolic acid, while trans-4-hydroxypipecolic acid and an unidentified compound were the major non-protein amino acids in B. prouacensis Aublet. The non-protein amino acid chemistry of these two species was therefore more similar to a representative of Swartzia examined, S. macrosema Harms, which also contained mono- and dihydroxypipecolic acids. The monotypic Candolleodendron brachystachyum (DC.) R.S. Cowan, considered related to Bocoa, accumulated trans-5-hydroxypipecolic acid. LC-MS data on flavonoids obtained from four of the extracts revealed the presence of flavone C-glycosides in B. viridiflora and B. prouacensis but only flavonoid O-glycosides in B. alterna and B. mollis. The chemical division of Bocoa concurs with studies of other character types and recent molecular phylogenies.
利用植物标本叶片碎片提取物，通过液相色谱-质谱联用（LC-MS）和气相色谱-质谱联用（GC-MS）技术，对豆科植物博科阿属（蝶形花亚科；斯瓦茨亚科）的非蛋白质氨基酸进行了调查。结果发现，交替博科阿（Benth.）R.S.考恩、欺骗博科阿R.S.考恩、利马博科阿R.S.考恩、软毛博科阿（Benth.）R.S.考恩和总状博科阿（胡伯）R.S.考恩含有2,4-亚甲基脯氨酸、2,4-亚甲基谷氨酸、顺式-1-氨基-3-羟甲基环丁烷-1-羧酸和δ-N-乙酰鸟氨酸。前三种化合物此前仅在阿泰莱亚属和杯盖豆属中被报道过，因此，这些结果支持了在最近的DNA序列数据系统发育分析中发现的与这些属的亲缘关系。相比之下，发现绿花博科阿（杜克）R.S.考恩含有反式-5-羟基哌啶酸和反式-4-顺式-5-二羟基哌啶酸，而反式-4-羟基哌啶酸和一种未鉴定的化合物是普劳阿森博科阿奥布莱特中主要的非蛋白质氨基酸。因此，这两个物种的非蛋白质氨基酸化学性质与所研究的斯瓦茨亚属的一个代表物种——大萼斯瓦茨哈姆斯更为相似，该物种也含有单羟基和二羟基哌啶酸。单型的短穗坎多勒odendron brachystachyum（DC.）R.S.考恩，被认为与博科阿属相关，积累了反式-5-羟基哌啶酸。从四种提取物中获得的关于黄酮类化合物的LC-MS数据显示，绿花博科阿和普劳阿森博科阿中存在黄酮C-糖苷，而交替博科阿和软毛博科阿中仅存在黄酮O-糖苷。博科阿属的化学分类与其他特征类型的研究以及最近的分子系统发育研究结果一致。
