牛膝叶对爱泼斯坦-巴尔病毒激活及二阶段小鼠皮肤致癌作用的癌症化学预防活性。

Cancer chemopreventive activity of Achyranthes aspera leaves on Epstein-Barr virus activation and two-stage mouse skin carcinogenesis.

作者信息

Chakraborty Asima, Brantner Adelheid, Mukainaka Teruo, Nobukuni Yoshitoshi, Kuchide Masashi, Konoshima Takao, Tokuda Harukuni, Nishino Hoyoku

机构信息

Institute of Pharmacognosy, University of Graz, Universitaetsplatz 4/I, A-8010 Graz, Austria.

出版信息

Cancer Lett. 2002 Mar 8;177(1):1-5. doi: 10.1016/s0304-3835(01)00766-2.

DOI:10.1016/s0304-3835(01)00766-2

PMID:11809524

Abstract

Achyranthes aspera leaves have been assessed for chemopreventive activity. The MeOH extract, alkaloid, non-alkaloid and saponin fractions exhibited significant inhibitory effects (concentration 100 microg) on the Epstein-Barr virus early antigen activation induced by the tumor promotor 12-O-tetradecanoylphorbol-13-acetate in Raji cells. In this in vitro assay the non-alkaloid fraction containing mainly non-polar compounds showed the most significant inhibitory activity (96.9%; 60% viability). In the in vivo two-stage mouse skin carcinogenesis test the total methanolic extract possessed a pronounced anticarcinogenic effect (76%). The present study suggests that A. aspera leaf extract and the non-alkaloid fraction are valuable antitumor promotors in carcinogenesis.

摘要

对牛膝叶的化学预防活性进行了评估。甲醇提取物、生物碱、非生物碱和皂苷组分对肿瘤启动子12-O-十四酰佛波醇-13-乙酸酯在Raji细胞中诱导的爱泼斯坦-巴尔病毒早期抗原激活表现出显著的抑制作用（浓度为100微克）。在该体外试验中，主要含有非极性化合物的非生物碱组分显示出最显著的抑制活性（96.9%；60%存活率）。在体内两阶段小鼠皮肤致癌试验中，总甲醇提取物具有显著的抗癌作用（76%）。本研究表明，牛膝叶提取物和非生物碱组分在致癌过程中是有价值的抗肿瘤启动剂。