Burckard J, Mazen A, Champagne M
Biochim Biophys Acta. 1975 Oct 20;405(2):434-41. doi: 10.1016/0005-2795(75)90108-7.
Those non-histone chromosomal proteins which are easily extractible from chick erythrocytes differ substantially from proteins similarly extracted from other tissues of various species. Although a protein P1 was isolated along with histone H1 by extraction of calf thymus chromatin with HC1O4, the same procedure did not extract this protein from chick erythrocyte chromatin of either normal or regenerating blood. Likewise , non-histone proteins extracted with 0.35 M NaCl from calf thymus differed from those of normal chick erythrocytes, which were qualitatively identical but quantitatively inferior to those of regenerating blood. The major protein of about 25 000 molecular weight, totally extracted with 0.35 M NaCl from calf thymus, was not found in chick erythrocyte chromatin, but rather another major protein of about 35 000 molecular weight was partially extracted from erythrocytes.
那些易于从小鸡红细胞中提取的非组蛋白染色体蛋白，与从不同物种的其他组织中以类似方式提取的蛋白有很大差异。虽然通过用高氯酸提取小牛胸腺染色质，可将蛋白P1与组蛋白H1一同分离出来，但相同的步骤却无法从正常或再生血液的小鸡红细胞染色质中提取出该蛋白。同样，用0.35M氯化钠从小牛胸腺中提取的非组蛋白与正常小鸡红细胞中的非组蛋白不同，正常小鸡红细胞中的非组蛋白在质量上相同，但在数量上比再生血液中的少。从小牛胸腺中用0.35M氯化钠完全提取出的约25000分子量的主要蛋白，在小鸡红细胞染色质中未被发现，相反，约35000分子量的另一种主要蛋白却能从小鸡红细胞中被部分提取出来。
