一种细菌鸟嘌呤核苷酸交换因子可激活嗜肺军团菌吞噬体上的ARF。
A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes.
作者信息
Nagai Hiroki, Kagan Jonathan C, Zhu Xinjun, Kahn Richard A, Roy Craig R
机构信息
Section of Microbial Pathogenesis, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, CT 06536, USA.
出版信息
Science. 2002 Jan 25;295(5555):679-82. doi: 10.1126/science.1067025.
The intracellular pathogen Legionella pneumophila subverts vesicle traffic in eukaryotic host cells to create a vacuole that supports replication. The dot/icm genes encode a protein secretion apparatus that L. pneumophila require for biogenesis of this vacuole. Here we show that L. pneumophila produce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases). The RalF protein is required for the localization of ARF on phagosomes containing L. pneumophila. Translocation of RalF protein through the phagosomal membrane is a dot/icm-dependent process. Thus, RalF is a substrate of the Dot/Icm secretion apparatus.
细胞内病原体嗜肺军团菌破坏真核宿主细胞中的囊泡运输，以形成支持其复制的液泡。dot/icm基因编码一种蛋白质分泌装置，嗜肺军团菌形成该液泡的生物合成需要该装置。我们在此表明，嗜肺军团菌产生一种名为RalF的蛋白质，它作为鸟苷三磷酸酶（GTP酶）的ADP核糖基化因子（ARF）家族的交换因子发挥作用。RalF蛋白是ARF定位于含有嗜肺军团菌的吞噬体所必需的。RalF蛋白通过吞噬体膜的转运是一个依赖于dot/icm的过程。因此，RalF是Dot/Icm分泌装置的底物。
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