Detera S D, Friedberg F
Int J Pept Protein Res. 1979 Oct;14(4):364-72. doi: 10.1111/j.1399-3011.1979.tb01945.x.
Bacillus subtilis alpha-amylase was cleaved with cyanogen bromide and the amino terminal sequences of the purified products were determined. The molecular weights of the cyanogen bromide fragments were ascertained on an agarose column equilibrated with 6 M guanidine hydrochloride. The molecular wieghts of these fragments were also calculated from their amino acid compositions. The data obtained bzyme monomer as 48,000.
用溴化氰裂解枯草芽孢杆菌α-淀粉酶，并测定纯化产物的氨基末端序列。在以6M盐酸胍平衡的琼脂糖柱上确定溴化氰片段的分子量。这些片段的分子量也根据其氨基酸组成进行计算。得到的数据表明该酶单体的分子量为48,000。
