Ajisaka H, Fushida S, Yonemura Y, Miwa K
Department of Surgery II, School of Medicine, Kanazawa University, Kanazawa, Japan.
Hepatogastroenterology. 2001 Nov-Dec;48(42):1788-92.
BACKGROUND/AIMS: Lymph node metastases are thought to be formed as lymphatic flow, so the first metastasis is formed in the lymph node nearest the primary tumor and then further lymph node metastases are formed continuously down stream. In this study of gastric cancer, we evaluated whether IGF-2 (insulin-like growth factor-2), c-MET (hepatocyte growth factor receptor), MMP-7 (matrix metalloproteinase-7) and MUC-1, which are associated with nodal metastasis from primary tumor, are concerned with metastasis from nodal metastatic lesion to other nodes.
Tissue specimens were obtained from 146 patients who underwent gastrectomy at the Department of Surgery II of Kanazawa University between 1989 and 1997. We evaluated the expression of IGF-2, c-MET, MMP-7 and MUC-1 in 146 primary tumors and 66 metastatic lymph nodes by immunohistological staining.
In primary lesions, these 4 factors had significant association with lymphatic vessel infiltration and MMP-7 and MUC-1 had significant association with nodal metastases. In nodal metastatic lesions, positive rates of MMP-7 and MUC-1 were higher than in primary lesions. Furthermore, 37 (73%) of the 51 cases with positive staining for MMP-7 in metastatic lesions in perigastric lymph nodes and 5 (33%) of the 15 cases without the staining had nodal metastases in group 2 or more, showing significant difference (P < 0.05). And 37 (76%) of the 49 cases with positive staining for MUC-1 in metastatic lesions in perigastric lymph nodes and 5 (29%) of the 17 cases without the staining had nodal metastases in group 2 or more, showing significant difference (P < 0.01).
MMP-7 and MUC-1 were associated with not only nodal metastasis from primary tumor but also metastasis from nodal metastatic lesion to other nodes.
背景/目的：淋巴结转移被认为是随着淋巴液流动形成的，所以首次转移在距离原发肿瘤最近的淋巴结形成，随后沿下游不断形成更多的淋巴结转移。在这项胃癌研究中，我们评估了与原发肿瘤淋巴结转移相关的胰岛素样生长因子-2（IGF-2）、肝细胞生长因子受体（c-MET）、基质金属蛋白酶-7（MMP-7）和黏蛋白-1（MUC-1）是否与淋巴结转移灶向其他淋巴结的转移有关。
组织标本取自1989年至1997年间在金泽大学第二外科接受胃切除术的146例患者。我们通过免疫组织化学染色评估了146例原发肿瘤和66个转移淋巴结中IGF-2、c-MET、MMP-7和MUC-1的表达。
在原发灶中，这4种因子与淋巴管浸润显著相关，MMP-7和MUC-1与淋巴结转移显著相关。在淋巴结转移灶中，MMP-7和MUC-1的阳性率高于原发灶。此外，胃周淋巴结转移灶中MMP-7染色阳性的51例患者中有37例（73%）以及未染色的15例患者中有5例（33%）出现2组或更多组淋巴结转移，差异有统计学意义（P<0.05）。胃周淋巴结转移灶中MUC-1染色阳性的49例患者中有37例（76%）以及未染色的17例患者中有5例（29%）出现2组或更多组淋巴结转移，差异有统计学意义（P<0.01）。
MMP-7和MUC-1不仅与原发肿瘤的淋巴结转移有关，还与淋巴结转移灶向其他淋巴结的转移有关。
