Biotinylated lithocholic acids for affinity chromatography of mammalian DNA polymerases alpha and beta.
作者信息
Watanabe Madoka, Hanashima Shinya, Mizushina Yoshiyuki, Yoshida Hiromi, Oshige Masahiko, Sakaguchi Kengo, Sugawara Fumio
机构信息
Department of Applied Biological Science, Frontier Research Center for Genomic Drug Discovery, Tokyo University of Science, Noda, Chiba 278-8510, Japan.
出版信息
Bioorg Med Chem Lett. 2002 Feb 11;12(3):287-90. doi: 10.1016/s0960-894x(01)00725-9.
Abstract
Biotinylated lithocholic acids have been synthesized. The compounds inhibited mammalian DNA polymerases alpha and beta with dose-dependent manner. The streptavidine columns conjugated with the synthetic biotinylated compounds chromatographed both two enzymes eluted by KCl solution at the different concentrations.
摘要
Zotero 插件