Garcia M Lourdes, Herreras J M, Dios E, Argueso P, Almaraz A
IOBA, Instituto de Oftalmobiologia Aplicada, Universidad de Valladolid, Spain.
Mol Vis. 2002 Jan 25;8:10-6.
To assess the sensitivity, specificity, and reliability of peroxidase labeled lectin staining in the diagnosis of fungal keratitis in an experimental rabbit model.
Fungal keratitis by Candida albicans, Aspergillus fumigatus, and Fusarium solani was induced in rabbits. WGA-peroxidase staining of 660 corneal sections was performed. Fungal staining was evaluated independently by two observers. The test sensitivity, specificity, and reliability indexes were calculated.
The sensitivity of the lectin staining test for Candida albicans was 100% (95% CI: 93.51-100.00), and specificity was 100% (95% CI: 93.51-100.00). The sensitivity of the test for Aspergillus fumigatus was 96.36% (95% CI: 86.46-99.35), and specificity was 100% (95% CI: 93.51-100.00). The sensitivity of the test for Fusarium solani was 96.36% (95% CI: 86.46-99.35) and specificity was 96.15% (95% CI: 85.74-99.31). There was also a high degree of test-retest and inter-rater concordance for all three fungi tested. The test-retest k reliability indexes were 0.9455, 0.9636, and 0.8879, for Candida albicans, Aspergillus fumigatus, and Fusarium solani, respectively. The inter-rater k reliability indexes were 0.9636, 0.9818, and 0.9252, for Candida albicans, Aspergillus fumigatus, and Fusarium solani, respectively.
WGA-peroxidase staining is a very sensitive, specific, and reliable test for the identification of fungi in an experimental rabbit model of fungal keratitis.
在实验性兔模型中评估过氧化物酶标记凝集素染色在真菌性角膜炎诊断中的敏感性、特异性和可靠性。
在兔中诱导白色念珠菌、烟曲霉和茄病镰刀菌引起的真菌性角膜炎。对660个角膜切片进行WGA-过氧化物酶染色。由两名观察者独立评估真菌染色情况。计算检测的敏感性、特异性和可靠性指标。
凝集素染色试验对白色念珠菌的敏感性为100%（95%置信区间：93.51 - 100.00），特异性为100%（95%置信区间：93.51 - 100.00）。对烟曲霉检测的敏感性为96.36%（95%置信区间：86.46 - 99.35），特异性为100%（95%置信区间：93.51 - 100.00）。对茄病镰刀菌检测的敏感性为96.36%（95%置信区间：86.46 - 99.35），特异性为96.15%（95%置信区间：85.74 - 99.31）。对于所检测的所有三种真菌，重测和观察者间的一致性程度也很高。白色念珠菌、烟曲霉和茄病镰刀菌的重测k可靠性指标分别为0.9455、0.9636和0.8879。白色念珠菌、烟曲霉和茄病镰刀菌的观察者间k可靠性指标分别为0.9636、0.9818和0.9252。
在真菌性角膜炎的实验性兔模型中，WGA-过氧化物酶染色是一种用于鉴定真菌的非常敏感、特异且可靠的检测方法。
