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Efficient transduction and selection of human T-lymphocytes with bicistronic Thy1/HSV1-TK retroviral vector produced by a human packaging cell line.用人包装细胞系产生的双顺反子Thy1/HSV1-TK逆转录病毒载体对人T淋巴细胞进行高效转导和筛选。
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[A retroviral bicistronic strategy for efficient coexpression of ALDH1 and mdr1].
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High-efficiency transduction and long-term gene expression with a murine stem cell retroviral vector encoding the green fluorescent protein in human marrow stromal cells.使用编码绿色荧光蛋白的鼠干细胞逆转录病毒载体在人骨髓基质细胞中进行高效转导和长期基因表达。
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Intramuscular injection of an adenoviral vector expressing hepatocyte growth factor facilitates hepatic transduction with a retroviral vector in mice.肌肉注射表达肝细胞生长因子的腺病毒载体可促进逆转录病毒载体在小鼠肝脏中的转导。
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引用本文的文献

1
Effects of electroporation on primary rat hepatocytes in vitro.电穿孔对原代大鼠肝细胞的体外作用。
World J Gastroenterol. 2002 Oct;8(5):893-6. doi: 10.3748/wjg.v8.i5.893.

本文引用的文献

1
Construction of retroviral vectors to induce a strong expression of human class interferon gene in human hepatocellular carcinoma cells in vitro.构建逆转录病毒载体以在体外人肝癌细胞中诱导人Ⅰ型干扰素基因的强表达。
World J Gastroenterol. 1997 Sep 15;3(3):139-42. doi: 10.3748/wjg.v3.i3.139.
2
Intrasplenic transplantation of allogeneic hepatocytes prolongs survival in anhepatic rats.同种异体肝细胞脾内移植可延长无肝大鼠的存活时间。
Hepatology. 1998 Nov;28(5):1365-70. doi: 10.1002/hep.510280527.
3
Fetal rat hepatocytes: isolation, characterization, and transplantation in the Nagase analbuminemic rats.胎鼠肝细胞：在长谷白蛋白缺乏大鼠中的分离、鉴定及移植
Transplantation. 1997 Nov 15;64(9):1240-8. doi: 10.1097/00007890-199711150-00003.
4
Loss and recovery of liver regeneration in rats with fulminant hepatic failure.暴发性肝衰竭大鼠肝脏再生的丧失与恢复
J Surg Res. 1997 Oct;72(2):112-22. doi: 10.1006/jsre.1997.5175.
5
Treatment of surgically induced acute liver failure by transplantation of conditionally immortalized hepatocytes.
Transplantation. 1997 Jun 15;63(11):1541-7. doi: 10.1097/00007890-199706150-00001.
6
Iron may induce both DNA synthesis and repair in rat hepatocytes stimulated by EGF/pyruvate.铁可能会在由表皮生长因子/丙酮酸刺激的大鼠肝细胞中诱导DNA合成及修复。
J Hepatol. 1997 Mar;26(3):650-8. doi: 10.1016/s0168-8278(97)80432-1.
7
Preclinical studies for cell transplantation: isolation of primate fetal hepatocytes, their cryopreservation, and efficient retroviral transduction.
Hum Gene Ther. 1997 Feb 10;8(3):267-74. doi: 10.1089/hum.1997.8.3-267.
8
Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure.
Transplantation. 1997 Feb 27;63(4):559-69. doi: 10.1097/00007890-199702270-00014.
9
Hepatic drug delivery and gene therapy.肝脏药物递送与基因治疗。
Hepatology. 1997 Feb;25(2):484-91. doi: 10.1002/hep.510250238.
10
Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes.成年大鼠肝细胞的长期培养，这些肝细胞经历多次细胞分裂并表达正常的实质细胞表型。
Am J Pathol. 1996 Feb;148(2):383-92.

Transduction of primary rat hepatocytes with bicistronic retroviral vector.

作者信息

Xie Qing, Liao Dan, Zhou Xia-Qiu, Qian Shu-Bing, Cheng Shi-Shu

出版信息

World J Gastroenterol. 2000 Oct;6(5):725-729. doi: 10.3748/wjg.v6.i5.725.

DOI:10.3748/wjg.v6.i5.725
PMID:11819682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4688851/
Abstract
摘要