Asb4、Ata3和Dcn是通过使用理化学研究所cDNA微阵列进行高通量筛选鉴定出的新型印记基因。
Asb4, Ata3, and Dcn are novel imprinted genes identified by high-throughput screening using RIKEN cDNA microarray.
作者信息
Mizuno Yosuke, Sotomaru Yusuke, Katsuzawa Yukiko, Kono Tomohiro, Meguro Makiko, Oshimura Mitsuo, Kawai Jun, Tomaru Yasuhiro, Kiyosawa Hidenori, Nikaido Itoshi, Amanuma Hiroshi, Hayashizaki Yoshihide, Okazaki Yasushi
机构信息
Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Yokohama 230-0045, Japan.
出版信息
Biochem Biophys Res Commun. 2002 Feb 8;290(5):1499-505. doi: 10.1006/bbrc.2002.6370.
Genes differentially expressed between parthenogenetic and androgenetic embryos are candidates for the identification of imprinted genes, which are expressed specifically from the maternal or paternal allele. To search for genes differentially expressed between parthenogenetic and androgenetic embryos, we used the RIKEN full-length enriched mouse cDNA microarray. The 25 candidates obtained included 8 known imprinted genes (such as IgfII, Snrpn, and Neuronatin) and 3 new ones--Asb4 (ankyrin repeat and SOCS box-containing protein 4), Ata3 (amino acid transport system A3), and Decorin--which were confirmed by using normal diploid embryos from the reciprocal F1 crosses of B6 and JF1 mice. The 25 candidates also included genes that showed no imprinting-associated expression in normal diploid embryos. We describe a feasible high-throughput method of screening for novel imprinted genes by using the RIKEN cDNA microarray.
孤雌生殖胚胎和雄核发育胚胎之间差异表达的基因是鉴定印记基因的候选基因，印记基因是从母本或父本等位基因特异性表达的。为了寻找孤雌生殖胚胎和雄核发育胚胎之间差异表达的基因，我们使用了RIKEN全长富集小鼠cDNA微阵列。获得的25个候选基因包括8个已知的印记基因（如IgfII、Snrpn和Neuronatin）和3个新基因——Asb4（含锚蛋白重复序列和SOCS盒蛋白4）、Ata3（氨基酸转运系统A3）和核心蛋白聚糖——通过使用B6和JF1小鼠正反交F1杂交的正常二倍体胚胎得到了证实。这25个候选基因还包括在正常二倍体胚胎中未显示出与印记相关表达的基因。我们描述了一种使用RIKEN cDNA微阵列筛选新印记基因的可行的高通量方法。
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