KCO1是慢液泡（SV）离子通道的一个组成部分。
KCO1 is a component of the slow-vacuolar (SV) ion channel.
作者信息
Schönknecht Gerald, Spoormaker Petra, Steinmeyer Ralf, Brüggeman Lioubov, Ache Peter, Dutta Rajiv, Reintanz Birgit, Godde Matthias, Hedrich Rainer, Palme Klaus
机构信息
Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Molekulare Pfanzenphysiologie und Biophysik, Universität Würzburg, 97082 Würzburg, Germany.
出版信息
FEBS Lett. 2002 Jan 30;511(1-3):28-32. doi: 10.1016/s0014-5793(01)03273-2.
The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels.
拟南芥双孔钾离子通道KCO1与绿色荧光蛋白融合，并在烟草原生质体中表达。显微镜分析显示液泡膜上有明亮的绿色荧光。RT-PCR实验表明KCO1在叶肉中表达。分离出拟南芥野生型和kco1基因敲除植株的液泡用于膜片钳分析。与野生型相比，kco1植株中叶肉细胞液泡的慢激活液泡（SV）通道介导的电流明显更小。这表明KCO1参与了SV通道的形成。
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