Marques Mello Luane, Silva-Vergara Mario Leon, Rodrigues Virmondes
Laboratório de Imunologia, Faculdade de Medicina do Triangulo Mineiro, Uberaba, Brazil.
Hum Immunol. 2002 Feb;63(2):149-54. doi: 10.1016/s0198-8859(01)00375-5.
Paracoccidiodomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis. In humans, the disease presents a broad spectrum of clinical manifestations, ranging from localized mucocutaneous lesions to a widespread manifestation with involving the mononuclear phagocyte system. In attempt to better understand the regulation of immune response during the infection, this study analyzed the production of regulatory and inflammatory cytokines in 25 infected patients and 19 health controls. Regulatory and inflammatory cytokines were analyzed in supernatants of peripheral blood mononuclear cells (PBMC) stimulated with mitogens or soluble P. brasiliensis antigens. A pattern of Th2 immune response was observed in patients, mainly attributed to a higher production of IL-4 and IL-5 than to a lower production of IFN-gamma. Patients with disseminated infection presented undetectable levels of IFN-gamma after antigen stimulation and high levels of IL-1beta, which were probably associated with the inflammatory reaction observed in multifocal infection.
副球孢子菌病(PCM)由双相真菌巴西副球孢子菌引起。在人类中,该疾病呈现出广泛的临床表现,从局部黏膜皮肤病变到累及单核吞噬细胞系统的广泛表现。为了更好地了解感染期间免疫反应的调节,本研究分析了25例感染患者和19名健康对照中调节性和炎性细胞因子的产生。在用丝裂原或可溶性巴西副球孢子菌抗原刺激的外周血单个核细胞(PBMC)上清液中分析调节性和炎性细胞因子。在患者中观察到Th2免疫反应模式,主要归因于IL-4和IL-5的产生较高,而不是IFN-γ的产生较低。播散性感染患者在抗原刺激后IFN-γ水平检测不到,IL-1β水平较高,这可能与多灶性感染中观察到的炎症反应有关。