Bozzi A, Reis B S, Goulart M I, Pereira M C N, Pedroso E P, Goes A M
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.
Immunol Lett. 2007 Nov 30;114(1):23-30. doi: 10.1016/j.imlet.2007.08.004. Epub 2007 Sep 11.
Memory T cell populations in patients with paracoccidioidomycosis (PCM) were analyzed before and after chemotherapy treatment. Peripheral blood mononuclear cells (PBMC) collected from patients infected by Paracoccidioides brasiliensis or from non-infected individuals were stimulated in vitro with either membrane and extra-cellular antigens (MEXO) or yeast cell antigen preparation (PbAg) of P. brasiliensis. An increase in the level of CD4(+) memory T cells was determined in PBMC from PCM patients before (NT) and after treatment (TR) and in those with PCM relapsed (RE) compared to that from non-infected controls (NINF). The CD8(+) memory T cells were increased in PBMC from RE patients stimulated with MEXO, but not in NT or TR. The distribution of memory B cells did not differ between NT and TR patients, while a significant elevation was determined in RE patients and higher antibody levels were also detected. The cytokine analysis showed low production of IFN-gamma by cells from RE patients compared with NT or TR patients. In contrast, high production of IL-4 was detected in NT and RE patients, and moderate levels were produced by RE patients. These results suggest that IFN-gamma production may participate in the maintenance of immunological memory in the acquired protection against P. brasiliensis infection and this data can contribute to future development of successful treatment of PCM to avoid relapsing.
对副球孢子菌病（PCM）患者化疗前后的记忆性T细胞群体进行了分析。从感染巴西副球孢子菌的患者或未感染个体中收集外周血单核细胞（PBMC），体外分别用巴西副球孢子菌的膜和细胞外抗原（MEXO）或酵母细胞抗原制剂（PbAg）进行刺激。与未感染对照（NINF）相比，测定了PCM患者治疗前（NT）、治疗后（TR）以及复发（RE）患者PBMC中CD4(+)记忆性T细胞水平的升高情况。在用MEXO刺激的RE患者的PBMC中，CD8(+)记忆性T细胞增加，但在NT或TR患者中未增加。NT和TR患者之间记忆性B细胞的分布没有差异，而RE患者中记忆性B细胞显著升高，并且还检测到更高的抗体水平。细胞因子分析显示，与NT或TR患者相比，RE患者细胞产生的IFN-γ较低。相反，在NT和RE患者中检测到IL-4的高产生，RE患者产生中等水平的IL-4。这些结果表明，IFN-γ的产生可能参与了在获得性抗巴西副球孢子菌感染保护中免疫记忆的维持，并且这些数据有助于未来成功治疗PCM以避免复发的发展。
