七种不同酶联免疫吸附测定法用于金黄色葡萄球菌菌血症血清学诊断的评估
Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia.
作者信息
Ryding U, Espersen F, Söderquist B, Christensson B
机构信息
Department of Infectious Diseases, Ostersund Hospital, Ostersund, Sweden.
出版信息
Diagn Microbiol Infect Dis. 2002 Jan;42(1):9-15. doi: 10.1016/s0732-8893(01)00311-x.
OBJECTIVE
Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis.
METHODS
Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. aureus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, alpha-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls.
RESULTS
Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid,alpha-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia.
目的
评估金黄色葡萄球菌抗体的血清学检测方法在区分单纯性和复杂性金黄色葡萄球菌菌血症、金黄色葡萄球菌与非金黄色葡萄球菌菌血症以及金黄色葡萄球菌与非金黄色葡萄球菌心内膜炎方面的能力。
方法
采用酶联免疫吸附测定（ELISA）法检测129例金黄色葡萄球菌菌血症患者（包括51例心内膜炎患者）、78例非金黄色葡萄球菌菌血症患者（包括27例心内膜炎患者）和100例发热非菌血症对照者血清中针对七种金黄色葡萄球菌抗原（肽聚糖、磷壁酸、金黄色葡萄球菌超声裂解物、完整金黄色葡萄球菌细胞、α毒素、脂肪酶和荚膜多糖）的IgG抗体。
结果
全细胞ELISA检测最为敏感。所有检测方法的特异性均较低。针对全细胞、磷壁酸、α毒素、脂肪酶和荚膜多糖的两种不同ELISA组合确实能够区分金黄色葡萄球菌与非金黄色葡萄球菌心内膜炎，但无法区分单纯性和复杂性金黄色葡萄球菌菌血症。
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