金黄色葡萄球菌感染患者对金黄色葡萄球菌全细胞、脂肪酶和葡萄球菌溶血素的抗体反应。

Antibody response to Staphylococcus aureus whole cell, lipase and staphylolysin in patients with S. aureus infections.

作者信息

Ryding U, Renneberg J, Rollof J, Christensson B

机构信息

Department of Infectious Diseases, Lund University Hospital, Sweden.

出版信息

FEMS Microbiol Immunol. 1992 Jan;4(2):105-10. doi: 10.1111/j.1574-6968.1992.tb04976.x.

DOI:10.1111/j.1574-6968.1992.tb04976.x

PMID:1547022

Abstract

Three assays to measure antibodies against Staphylococcus aureus whole cells, lipase and staphylolysin were used to try to discriminate between complicated and uncomplicated S. aureus septicaemia. Sera were examined from 8 patients with S. aureus endocarditis, 23 patients with complicated S. aureus septicaemia, 12 patients with uncomplicated S. aureus septicaemia and 93 febrile non-septicaemic controls. No single assay could distinguish between complicated and uncomplicated S. aureus septicaemia. If the criterion for a positive result is defined as positive antibody level in the anti-lipase ELISA as well as in at least 1 of the other 2 assays, 10/31 patients with S. aureus endocarditis or complicated septicaemia were positive compared to 0/93 non-septicaemic patients and 0/12 patients with uncomplicated S. aureus septicaemia. Therefore, the combined use of serological assays in the diagnosis of complicated S. aureus septicaemia, one of which is the anti-lipase ELISA, is recommended.

摘要

采用三种检测方法来测定抗金黄色葡萄球菌全细胞、脂肪酶和葡萄球菌溶血素的抗体，试图区分复杂性和非复杂性金黄色葡萄球菌败血症。检测了8例金黄色葡萄球菌心内膜炎患者、23例复杂性金黄色葡萄球菌败血症患者、12例非复杂性金黄色葡萄球菌败血症患者以及93例发热非败血症对照者的血清。没有单一的检测方法能够区分复杂性和非复杂性金黄色葡萄球菌败血症。如果将阳性结果的标准定义为抗脂肪酶ELISA以及其他2种检测方法中至少1种检测方法的抗体水平为阳性，那么10/31例金黄色葡萄球菌心内膜炎或复杂性败血症患者呈阳性，相比之下，93例非败血症患者中0例呈阳性，12例非复杂性金黄色葡萄球菌败血症患者中0例呈阳性。因此，建议联合使用血清学检测方法来诊断复杂性金黄色葡萄球菌败血症，其中一种检测方法为抗脂肪酶ELISA。