通过生物合成掺入的特异性标记脂质的氘磁共振确定的莱氏无胆甾原体膜中的分子有序性。

Molecular order in Acholeplasma laidlawii membranes as determined by deuterium magnetic resonance of biosynthetically-incorporated specifically-labelled lipids.

作者信息

Stockton G W, Johnson K G, Butler K W, Polnaszek C F, Cyr R, Smith I C

出版信息

Biochim Biophys Acta. 1975 Sep 2;401(3):535-9. doi: 10.1016/0005-2736(75)90251-5.

Abstract

The first application of deuterium magentic resonance of specifically labelled lipids to the study of a natural biological membrane is described. Palmitic acid labelled at the terminal methyl group with deuterium was incorporated biosynthetically into the lipids of the plasma membrane of Acholeplasma laidlawii. The deuterium nuclear magnetic resonance spectra contain quadrupole splittings which yield directly order parameters for this region of the membrane. Below the growth temperature (37 degrees C) the spectra are indicative of lipid in both gel and liquid crystalline states. Above this temperature they demonstrate the existence of an entirely liquid crystalline membrane whose order parameter decreases rapidly with increasing temperature. Comparison with egg phosphatidylcholine over the same temperature range shows a more rapid change in order with temperature for the A. laidlawii membranes.

摘要

本文描述了将特定标记脂质的氘磁共振首次应用于天然生物膜研究的情况。用氘标记末端甲基的棕榈酸通过生物合成掺入莱氏无胆甾原体质膜的脂质中。氘核磁共振谱包含四极分裂，可直接得出该膜区域的序参数。在生长温度（37摄氏度）以下，谱图表明脂质处于凝胶态和液晶态。高于此温度时，谱图显示存在完全为液晶态的膜，其序参数随温度升高而迅速降低。在相同温度范围内与鸡蛋磷脂酰胆碱进行比较表明，莱氏无胆甾原体膜的序随温度变化更快。