Ogasawara N, Goto H, Watanabe T
Biochim Biophys Acta. 1975 Oct 22;403(2):530-7. doi: 10.1016/0005-2744(75)90081-9.
Three AMP deaminase isozymes (EC 3.5.4.6 AMP aminohydrolase) were purified from rat heart, kidney and muscle. These enzyme preparations contained only the required isozyme. Antisera to individual isozymes were prepared and immunological relationships were tested. There was no cross-reactivity as tested by precipitation experiments. The antisera precipitated only the corresponding isozyme and there was no effect on other isozymes. These isozymes were also different in Km values for AMP and in substrate specificity. From the present studies, combined with previous results, it seems clear that the heart, kidney and muscle enzymes are different basic types. It is proposed that the muscle enzyme be designated as AMP deaminase A; the enzyme in kidney and liver, AMP deaminase B; the enzyme in heart, AMP deaminase C. Brain extracts contained five isozymes; two parent isozymes (B and C) and presumably their three hybrids.
从大鼠心脏、肾脏和肌肉中纯化出三种AMP脱氨酶同工酶（EC 3.5.4.6 AMP氨基水解酶）。这些酶制剂仅含有所需的同工酶。制备了针对单个同工酶的抗血清，并测试了免疫关系。通过沉淀实验测试，没有交叉反应。抗血清仅沉淀相应的同工酶，对其他同工酶没有影响。这些同工酶在AMP的Km值和底物特异性方面也有所不同。根据目前的研究以及先前的结果，心脏、肾脏和肌肉中的酶似乎是不同的基本类型。建议将肌肉中的酶命名为AMP脱氨酶A；肾脏和肝脏中的酶命名为AMP脱氨酶B；心脏中的酶命名为AMP脱氨酶C。脑提取物含有五种同工酶；两种亲本同工酶（B和C）以及大概它们的三种杂种。
