Zarghi A, Dadashzadeh S, Kiayi Z
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Shaheed Beheshti, University of Medical Sciences, Tehran, Iran.
Boll Chim Farm. 2001 Nov-Dec;140(6):458-61.
A simple and selective high-performance liquid chromatographic method for the determination of amitriptyline in human plasma has been developed. For plasma samples, the protein was removed with 1 M NaOH and 0.7 M ZnSO4. aqueous solutions. The chromatographic separation was performed on an analytical mbondapak C18 column (250 3.9 mm, i.d) with an isocratic mobile phase consisting of phosphate buffer-acetonitrile-triethylamine (65:35:0.1 v/v/v) adjusted to pH 5.1. Clomipramine was used as an internal standard. Using ultraviolet detection at 239 nm, the detection limit for amitriptyline in plasma was 5 ng/ml. No interferences were found with tricyclic antidepressant drugs, which allows this method to be used in clinical studies. The calibration curve was linear over the concentration range 5-200 ng/ml. The recovery was complete for plasma. The inter-day and intra-day assay coefficients of variation were found to be less than 10%.
已开发出一种简单且选择性高的高效液相色谱法用于测定人血浆中的阿米替林。对于血浆样品，用1 M氢氧化钠和0.7 M硫酸锌水溶液去除蛋白质。色谱分离在分析型mbondapak C18柱（250×3.9 mm，内径）上进行，等度流动相由磷酸盐缓冲液 - 乙腈 - 三乙胺（65:35:0.1 v/v/v）组成，pH调至5.1。氯米帕明用作内标。采用239 nm紫外检测，血浆中阿米替林的检测限为5 ng/ml。三环类抗抑郁药未发现干扰，这使得该方法可用于临床研究。校准曲线在5 - 200 ng/ml浓度范围内呈线性。血浆回收率良好。日间和日内测定变异系数均小于10%。
