Marrazzo W N, Merson R L, McCoy B J
Biotechnol Bioeng. 1975 Oct;17(10):1515-28. doi: 10.1002/bit.260171010.
To describe axial dispersion, particle film mass transfer, intraparticle diffusion, and the chemical reaction of the substrate for enzymes immobilized in porous particles in packed columns, we have developed mathematical models for first- and zero-order limits of Michaelis-Menten kinetics. Steady-state solutions were derived for both long and short column boundary conditions and for plug flow. Theory was compared to experiments by hydrolysis of sucrose catalyzed by invertase bound to porous glass particles. Steady-state conversions were measured for a range of flow rates. Pulse response experiments with inert packing were used to determine values of bed void fraction and particle porosity.
为了描述填充柱中固定在多孔颗粒内的酶的轴向扩散、颗粒膜传质、颗粒内扩散以及底物的化学反应，我们针对米氏动力学的一级和零级极限情况建立了数学模型。推导了长柱和短柱边界条件以及平推流情况下的稳态解。通过与结合在多孔玻璃颗粒上的转化酶催化蔗糖水解的实验对理论进行了比较。测量了一系列流速下的稳态转化率。使用惰性填料的脉冲响应实验来确定床层空隙率和颗粒孔隙率的值。
