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[实验大鼠卵子发生中的自发染色体畸变]

[Spontaneous chromosome aberrations in the oogenesis of laboratory rats].

作者信息

Dyban A P, Chebotar' N A

出版信息

Biull Eksp Biol Med. 1975 Aug;80(8):103-6.

PMID:1182291
Abstract

Cytological preparations were made by Tarkovsky's method from 2335 rat oocytes obtained after an induced superodulation. The chromosomes could be counted exactly in 861 oocytes. In 797 oocytes (92.7%) euploidy (metaphase II with 21 chromosomes) and in 64 oocytes (7.5%) aneuploidy was found. 60 oocytes were hypoploid, but only 4 oocytes (0.4%) were hyperploid (with 22 chromosomes). Hypoploidy can often be due to the presence of artefacts. Probably the rate of spontaneous aneuploidy in rat oogenesis is about 0.8%, this being significantly lower than the rate of spontaneous aneuploidy in mice oogenesis.

摘要

采用塔尔科夫斯基方法,从诱导超排卵后获得的2335个大鼠卵母细胞制备细胞涂片。在861个卵母细胞中可以准确计数染色体。在797个卵母细胞(92.7%)中发现整倍体(有21条染色体的中期II),在64个卵母细胞(7.5%)中发现非整倍体。60个卵母细胞为亚二倍体,但只有4个卵母细胞(0.4%)为超二倍体(有22条染色体)。亚二倍体常常可能是由于人为因素的存在。大鼠卵子发生中自发非整倍体的发生率可能约为0.8%,这明显低于小鼠卵子发生中自发非整倍体的发生率。

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1
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Biull Eksp Biol Med. 1975 Aug;80(8):103-6.
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