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美国白蛾(鳞翅目,昆虫纲)幼虫咽侧体的超微结构

Ultrastructure of the larval corpus allatum of hyphantria cunea Drury (Insecta, Lepidoptera).

作者信息

Melnikova E J, Panov A A

出版信息

Cell Tissue Res. 1975 Oct 13;162(3):395-410. doi: 10.1007/BF00220186.

DOI:10.1007/BF00220186
PMID:1182771
Abstract

The corpora allata of the three last larval instars were studied in newly molted animals, at the beginning, middle, and end of the feeding period, and during the molt period. They were found to consist of uniform gland cells, whose ultrastructure changes in the course of the instars. In glands cells considered to be resting, the outer and inner nuclear membranes run in parallel without forming a dilated perinuclear space. Mitochondria are small, polymorphic, with an electron-dense matrix. The smooth endoplasmic reticulum (SER) appears as stacks of parallel cisternae near the nuclear envelope and in the rest of the cytoplasm, and as accumulations of twisted profiles. Occasionally, the SER takes the form of paracrystalline bodies. There are few small smooth-surfaced vesicles in the cytoplasm. In cells considered as active, a dilated perinuclear space occurs. The peripheral ends of profiles forming the SER are swollen, and numerous vesicles and vacuoles bud off from them to fill the cytoplasm. Mitochondria are large, with a more transparent matrix. The plasma membrane of gland cells located just beneath the connective tissue sheath forms numerous small invaginations. The corpora allata consist of resting cells during the molt periods. At the beginning of each instar, few active gland cells appear. In the middle of the second to last and the third to last instars, the bulk of the gland cells is active. At the end of these instars, there are both active and inactive cells. In the middle of the last instar, the gland cells are inactive or subactive, and at its end, all gland cells are completely inactive.

摘要

对最后三个幼虫龄期新蜕皮动物的咽侧体进行了研究,研究时间为取食期开始、中期、末期以及蜕皮期。发现咽侧体由均匀的腺细胞组成,其超微结构在龄期过程中会发生变化。在被认为处于静止状态的腺细胞中,外核膜和内核膜平行排列,未形成扩张的核周间隙。线粒体小,形态多样,基质电子密度高。滑面内质网(SER)在核膜附近和细胞质其余部分表现为平行排列的扁平囊堆叠,以及扭曲轮廓的聚集。偶尔,SER呈副晶体形式。细胞质中很少有小的光滑表面囊泡。在被认为活跃的细胞中,会出现扩张的核周间隙。构成SER的轮廓外周端肿胀,许多囊泡和液泡从它们上芽生出来填充细胞质。线粒体大,基质更透明。位于结缔组织鞘下方的腺细胞的质膜形成许多小凹陷。蜕皮期咽侧体由静止细胞组成。在每个龄期开始时,出现少量活跃的腺细胞。在倒数第二龄期和倒数第三龄期的中期,大部分腺细胞是活跃的。在这些龄期结束时,既有活跃细胞也有不活跃细胞。在最后一龄期的中期,腺细胞不活跃或亚活跃,在其末期,所有腺细胞完全不活跃。

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