Limjoco T, Nihrane A, Silver J
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Virology. 1995 Apr 1;208(1):75-83. doi: 10.1006/viro.1995.1131.
Mice and chickens that inherit certain retroviral envelope genes are resistant to infection with related retroviruses. Previously, we described two transgenic mouse strains bearing a retroviral envelope gene, Fv4, that confers resistance to infection with ecotropic retroviruses (T. I. Limjoco et al., 1993, J. Virol. 67, 4163-4168). Here, we present results with these and an additional transgenic strain that show that (1) the level of resistance is correlated with level of expression of the transgene, (2) low-level expression of the transgene is associated with an unexpected and possibly immune-mediated phenotype of recovery from viremia, (3) resistance can be transferred by bone marrow transplantation and is "dominant" in chimeras containing mixtures of transgenic resistant plus control bone marrow, and (4) transplantation after infection with Friend Virus is much less effective than transplantation before infection. We discuss the implications of these results for gene therapy of retroviral infection.
继承某些逆转录病毒包膜基因的小鼠和鸡对相关逆转录病毒感染具有抗性。此前，我们描述了两种携带逆转录病毒包膜基因Fv4的转基因小鼠品系，该基因赋予了对嗜亲性逆转录病毒感染的抗性（T.I. Limjoco等人，1993年，《病毒学杂志》67卷，4163 - 4168页）。在此，我们展示了这些以及另一个转基因品系的结果，表明：（1）抗性水平与转基因的表达水平相关；（2）转基因的低水平表达与从病毒血症中恢复的一种意想不到且可能是免疫介导的表型相关；（3）抗性可通过骨髓移植转移，并且在含有转基因抗性骨髓与对照骨髓混合物的嵌合体中是“显性的”；（4）感染弗氏病毒后进行移植比感染前移植效果差得多。我们讨论了这些结果对逆转录病毒感染基因治疗的意义。
