[自体牙周膜细胞移植对犬牙周组织再生的影响]
[Effects of autogenous periodontal ligament cell transplantation on periodontal tissue regeneration in dogs].
作者信息
Ou L, Liu H, Pang J
机构信息
Department of Stomatology, General Hospital of PLA, Beijing 100853, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2000 Jan;35(1):44-6.
OBJECTIVE
To evaluate the effect of autogenous periodontal ligament cell (PDL) transplantation in periodontal tissue regeneration.
METHODS
PDL cells derived from the same dog were cultured with alpha-MEM. 1 x 10(5) cells of first passage were allowed to attach to the collagen membrane for 24 hours. The membrane-cells were transplanted into periodontal defect in the same dog. Then the defects were covered with e-PTFE membranes. The defects covered only with e-PTFE were the controls. Eighteen teeth of 6 dogs for each group were included. The dogs were sacrificed after 6 weeks.
RESULTS
The results showed that new bone formation (4.00 +/- 0.13) mm in test group was significantly higher than that of in control group (3.09 +/- 0.28) mm, P < 0.05. The new cementum formation in test group was better than control group (P < 0.05).
CONCLUSION
The results suggested that PDL cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration in dogs.
目的
评估自体牙周膜细胞（PDL）移植对牙周组织再生的影响。
方法
从同一只犬获取的牙周膜细胞用α-MEM培养基培养。将第一代的1×10(5)个细胞接种于胶原膜上24小时。将带有细胞的胶原膜移植到同一只犬的牙周缺损处。然后用e-PTFE膜覆盖缺损处。仅用e-PTFE膜覆盖的缺损作为对照组。每组包括6只犬的18颗牙齿。6周后处死实验犬。
结果
结果显示，实验组新骨形成（4.00±0.13）mm，显著高于对照组（3.09±0.28）mm，P<0.05。实验组新牙骨质形成优于对照组（P<0.05）。
结论
结果表明，引导组织再生技术联合牙周膜细胞移植可促进犬牙周组织再生。
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