[β-连环蛋白及其mRNA在肾细胞癌中的表达]
[Beta-catenin and its mRNA in renal cell carcinoma].
作者信息
Zang T, Zhuang L, Zhang Z, Xin D, Guo Y
机构信息
Institute of Urology, Beijing Medcal University, Beijing 100034, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2000 Jan;38(1):37-9.
OBJECTIVES
To investigate the role of beta-catenin in renal cell carcinoma.
METHODS
The expression of beta-catenin in 26 cases of renal cell carcinoma was studied by LSAB immunohistochemistry, Western blotting and RT-PCR.
RESULTS
The expression of beta-catenin was higher in cancer tissues than in normal kidney tissues in 25 cases and the level of beta-catenin was associated with tumor stage. The expression was obviously higher in pT3 and pT4 than in pT1 and pT2 (P < 0.01). The expression of its mRNA was not higher in tumor cells than in normal cells. Beta-catenin was expressed mainly in cytoplasm.
CONCLUSIONS
Beta-catenin might be related to the occurrence and development of kidney tumor.
目的
探讨β-连环蛋白在肾细胞癌中的作用。
方法
采用LSAB免疫组化、蛋白质印迹法及逆转录聚合酶链反应（RT-PCR）研究26例肾细胞癌中β-连环蛋白的表达情况。
结果
25例患者癌组织中β-连环蛋白的表达高于正常肾组织，且β-连环蛋白水平与肿瘤分期相关。pT3和pT4期的表达明显高于pT1和pT2期（P<0.01）。其mRNA在肿瘤细胞中的表达并不高于正常细胞。β-连环蛋白主要在细胞质中表达。
结论
β-连环蛋白可能与肾肿瘤的发生发展有关。
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