Pi Xiujun, Voogt James L, Grattan David R
Department of Molecular and Integrative Physiology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160-7401, USA.
J Neurosci Res. 2002 Feb 15;67(4):551-8. doi: 10.1002/jnr.10147.
The observation of prolactin modulation of the nigrostriatal dopaminergic system suggests the expression of prolactin receptor in the corpus striatum or substantia nigra. The present study investigated expression of prolactin receptor mRNA in tissues microdissected from the corpus striatum and substantia nigra of the rat. By using reverse transcription PCR combined with Southern hybridization, the long form of prolactin receptor mRNA was detected in the substantia nigra, caudate putamen, globus pallidus, and ventral pallidum in ovariectomized rats, whereas the short form was not detectable in any of these areas. Estrogen had no effect on expression of the long-form mRNA in the substantia nigra and corpus striatum. By using the RNase protection assay, the expression of both short and long forms of prolactin receptor mRNA was observed in the corpus striatum in ovariectomized rats. Again, levels of expression were not significantly altered by estrogen treatment. Both forms of prolactin receptor mRNA were clearly expressed in the choroid plexus and were up-regulated by estrogen treatment. The expression of both forms of prolactin receptor mRNA in nigrostriatal areas may help to support the hypothesis that prolactin has direct actions on these brain regions.
对催乳素对黑质纹状体多巴胺能系统调节作用的观察表明，催乳素受体在纹状体或黑质中表达。本研究调查了从大鼠纹状体和黑质显微切割得到的组织中催乳素受体mRNA的表达情况。通过逆转录PCR结合Southern杂交，在去卵巢大鼠的黑质、尾状壳核、苍白球和腹侧苍白球中检测到了催乳素受体mRNA的长形式，而在这些区域中的任何一个都未检测到短形式。雌激素对黑质和纹状体中长形式mRNA的表达没有影响。通过核糖核酸酶保护分析，在去卵巢大鼠的纹状体中观察到了催乳素受体mRNA短形式和长形式的表达。同样，雌激素处理并未显著改变表达水平。催乳素受体mRNA的两种形式在脉络丛中均有明显表达，且经雌激素处理后上调。黑质纹状体区域中催乳素受体mRNA两种形式的表达可能有助于支持催乳素对这些脑区有直接作用的假说。
