Krajewska M, Mai J K, Zapata J M, Ashwell K W S, Schendel S L, Reed J C, Krajewski S
The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA.
Cell Death Differ. 2002 Feb;9(2):145-57. doi: 10.1038/sj.cdd.4400934.
We have used immunohistochemistry and immunoblotting to examine the expression of Bid and four other Bcl-2 family proteins (Bcl-2, Bcl-X, Bax and Bak) in the developing and adult murine central nervous system (CNS). Bid protein is widespread in embryonic and postnatal brain, and its expression is maintained at a high level late into the adulthood. Bid is expressed both in the germ disc, early neural tube, proliferating stem cells of ventricular zones, and in postmitotic, differentiated neurons of the developing central and peripheral nervous system. As the differentiation proceeds, the neurons express higher levels of Bid than the stem cells of the paraventricular zone. Both in embryonic and postnatal life, Bid protein is present in the most vital regions of brain, such as the limbic system, basal ganglia, mesencephalic tectum, Purkinje cells in cerebellum, and the ventral columns of spinal cord. The p15 cleaved form of Bid was detectable in the brain specimens at fetal stages of development, consistent with caspase-mediated activation of this pro-apoptotic Bcl-2 family protein. Among the Bcl-2 family proteins only Bid and Bcl-XL continue to be expressed at high levels in the adult brain.
我们运用免疫组织化学和免疫印迹法，检测了Bid及其他四种Bcl-2家族蛋白（Bcl-2、Bcl-X、Bax和Bak）在发育中和成年小鼠中枢神经系统（CNS）中的表达情况。Bid蛋白在胚胎期和出生后的大脑中广泛存在，其表达在成年后期仍维持在较高水平。Bid在胚盘、早期神经管、脑室区增殖的干细胞以及发育中的中枢和外周神经系统有丝分裂后分化的神经元中均有表达。随着分化的进行，神经元中Bid的表达水平高于室旁区的干细胞。在胚胎期和出生后的生命过程中，Bid蛋白存在于大脑中最重要的区域，如边缘系统、基底神经节、中脑顶盖、小脑浦肯野细胞以及脊髓腹侧柱。在发育的胎儿阶段的脑标本中可检测到Bid的p15裂解形式，这与该促凋亡Bcl-2家族蛋白的半胱天冬酶介导的激活一致。在Bcl-2家族蛋白中，只有Bid和Bcl-XL在成年大脑中继续高水平表达。
