Vidal S, Morante M P, Mosquera A M, Moga E, Querol S, García J, Rodríguez-Sánchez J L
Department of Immunology, Hospital Sant Pau and Inst. Rec. Sant Pau, Barcelona, Spain.
Tissue Antigens. 2001 Nov;58(5):343-4. doi: 10.1034/j.1399-0039.2001.580510.x.
We report a novel HLA-B41 allele (HLA-B4106) initially detected by an unusual sequence-specific oligonucleotide (SSO) hybridization pattern and identified by sequence-based HLA typing. Molecular cloning and sequencing determined that the new HLA-B allele was identical to the HLA-B4101 in exon 2 and 3 except for a single nucleotide substitution in the exon 3 changing codon 204 from Glu to Gln (GAG-->CAG). In addition, intron 2 was identical to the published HLA-B4104 intron 2, except for the base 509 (C-->G).
我们报告了一种新型的HLA - B41等位基因（HLA - B4106），最初通过异常的序列特异性寡核苷酸（SSO）杂交模式检测到，并通过基于序列的HLA分型进行鉴定。分子克隆和测序确定，新的HLA - B等位基因在第2和第3外显子上与HLA - B4101相同，除了第3外显子中有一个单核苷酸替换，导致密码子204由Glu变为Gln（GAG→CAG）。此外，内含子2与已发表的HLA - B4104内含子2相同，除了第509位碱基（C→G）。
