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大肠杆菌色氨酸操纵子中的衰减作用：涉及色氨酸密码子区域的RNA二级结构的作用。

Attenuation in the Escherichia coli tryptophan operon: role of RNA secondary structure involving the tryptophan codon region.

作者信息

Oxender D L, Zurawski G, Yanofsky C

出版信息

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5524-8. doi: 10.1073/pnas.76.11.5524.

DOI:10.1073/pnas.76.11.5524
PMID:118451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411681/
Abstract

The secondary structure of the terminated trp leader transcript from Escherichia coli was analyzed by RNase T1 partial digestion. Base-paired regions were recovered by nondenaturing gel electrophoresis and identified by denaturing gel electrophoresis and fingerprinting. The tandem tryptophan codons in the leader peptide coding region were found to be base paired with a more distal region of the transcript. This and other secondary structures that the trp leader RNA can form help explain the physiological response of the operon as well as the behavior of regulatory mutants.

摘要

通过核糖核酸酶T1部分消化分析了来自大肠杆菌的终止色氨酸前导转录物的二级结构。通过非变性凝胶电泳回收碱基配对区域，并通过变性凝胶电泳和指纹图谱进行鉴定。发现前导肽编码区域中的串联色氨酸密码子与转录物的更远端区域碱基配对。色氨酸前导RNA可以形成的这种以及其他二级结构有助于解释操纵子的生理反应以及调节突变体的行为。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/ac3c91580d4a/pnas00011-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/868b4623bada/pnas00011-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/3590df68cdee/pnas00011-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/ac3c91580d4a/pnas00011-0119-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/868b4623bada/pnas00011-0119-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/3590df68cdee/pnas00011-0119-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/013b/411681/ac3c91580d4a/pnas00011-0119-c.jpg

相似文献

1
Attenuation in the Escherichia coli tryptophan operon: role of RNA secondary structure involving the tryptophan codon region.大肠杆菌色氨酸操纵子中的衰减作用：涉及色氨酸密码子区域的RNA二级结构的作用。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5524-8. doi: 10.1073/pnas.76.11.5524.
2
The effects of leader peptide sequence and length on attenuation control of the trp operon of E.coli.前导肽序列和长度对大肠杆菌色氨酸操纵子衰减调控的影响。
Nucleic Acids Res. 1991 Feb 25;19(4):795-800. doi: 10.1093/nar/19.4.795.
3
MtrB from Bacillus subtilis binds specifically to trp leader RNA in a tryptophan-dependent manner.来自枯草芽孢杆菌的MtrB以色氨酸依赖的方式特异性结合色氨酸前导RNA。
Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):128-32. doi: 10.1073/pnas.90.1.128.
4
Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon.大肠杆菌色氨酸操纵子弱化子处转录终止的翻译调控
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5988-92. doi: 10.1073/pnas.75.12.5988.
5
Mutations replacing the leucine codons or altering the length of the amino acid-coding portion of the ilvGMEDA leader region of Escherichia coli.替换大肠杆菌ilvGMEDA前导区亮氨酸密码子或改变其氨基酸编码部分长度的突变。
J Bacteriol. 1991 Apr;173(7):2341-53. doi: 10.1128/jb.173.7.2341-2353.1991.
6
Replacement of the Escherichia coli trp operon attenuation control codons alters operon expression.替换大肠杆菌色氨酸操纵子的衰减控制密码子会改变操纵子的表达。
J Mol Biol. 1990 Nov 5;216(1):25-37. doi: 10.1016/S0022-2836(05)80058-0.
7
Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.大肠杆菌色氨酸操纵子前导区中的单碱基对改变可解除色氨酸衰减子处的转录终止。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4833-7. doi: 10.1073/pnas.75.10.4833.
8
Transcription termination in vitro at the tryptophan operon attenuator is controlled by secondary structures in the leader transcript.色氨酸操纵子衰减子在体外的转录终止由前导转录本中的二级结构控制。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2206-10. doi: 10.1073/pnas.80.8.2206.
9
Genetic analysis of the tryptophan operon regulatory region using site-directed mutagenesis.利用定点诱变技术对色氨酸操纵子调控区域进行遗传分析。
J Mol Biol. 1984 May 25;175(3):299-312. doi: 10.1016/0022-2836(84)90350-4.
10
Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli.大肠杆菌苯丙氨酸操纵子前导区的核苷酸序列。
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271-5. doi: 10.1073/pnas.75.9.4271.

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本文引用的文献

1
Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan.在部分纯化的阻遏物和色氨酸存在的情况下，利用纯化的RNA聚合酶对色氨酸操纵子进行体外转录调控。
Nat New Biol. 1973 Oct 3;245(144):133-7. doi: 10.1038/newbio245133a0.
2
Improved estimation of secondary structure in ribonucleic acids.核糖核酸二级结构的改进估计。
Nat New Biol. 1973 Nov 14;246(150):40-1. doi: 10.1038/newbio246040a0.
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Stability of ribonucleic acid double-stranded helices.核糖核酸双链螺旋的稳定性
BMC Microbiol. 2022 Feb 11;22(1):52. doi: 10.1186/s12866-022-02453-w.
4
The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.稳定核糖核蛋白的世界及其通过梯度测序和相关方法的图谱绘制。
Front Mol Biosci. 2021 Apr 7;8:661448. doi: 10.3389/fmolb.2021.661448. eCollection 2021.
5
Complementary Tendencies in the Use of Regulatory Elements (Transcription Factors, Sigma Factors, and Riboswitches) in Bacteria and Archaea.细菌和古菌中调控元件（转录因子、σ 因子和核糖开关）使用的互补趋势。
J Bacteriol. 2020 Dec 18;203(2). doi: 10.1128/JB.00413-20.
6
RNA Polymerase's Relationship with the Ribosome: Not So Physical, Most of the Time.RNA 聚合酶与核糖体的关系：大多数情况下并非直接物理作用。
J Mol Biol. 2020 Jun 26;432(14):3981-3986. doi: 10.1016/j.jmb.2020.03.018. Epub 2020 Mar 19.
7
Regulation of Bacterial Gene Expression by Transcription Attenuation.转录衰减调控细菌基因表达。
Microbiol Mol Biol Rev. 2019 Jul 3;83(3). doi: 10.1128/MMBR.00019-19. Print 2019 Aug 21.
8
Modernized Tools for Streamlined Genetic Manipulation and Comparative Study of Wild and Diverse Proteobacterial Lineages.用于简化遗传操作和比较研究野生和多样变形杆菌谱系的现代化工具。
mBio. 2018 Oct 9;9(5):e01877-18. doi: 10.1128/mBio.01877-18.
9
Metabolic regulation is sufficient for global and robust coordination of glucose uptake, catabolism, energy production and growth in Escherichia coli.代谢调控足以实现大肠杆菌中葡萄糖摄取、分解代谢、能量产生及生长的全局且稳健的协调。
PLoS Comput Biol. 2017 Feb 10;13(2):e1005396. doi: 10.1371/journal.pcbi.1005396. eCollection 2017 Feb.
10
Gene- and genome-based analysis of significant codon patterns in yeast, rat and mice genomes with the CUT Codon UTilization tool.利用CUT密码子使用工具对酵母、大鼠和小鼠基因组中显著密码子模式进行基于基因和基因组的分析。
Methods. 2016 Sep 1;107:98-109. doi: 10.1016/j.ymeth.2016.05.010. Epub 2016 May 28.
J Mol Biol. 1974 Jul 15;86(4):843-53. doi: 10.1016/0022-2836(74)90357-x.
4
Interaction of the trp repressor and RNA polymerase with the trp operon.色氨酸阻遏物与RNA聚合酶和色氨酸操纵子的相互作用。
J Mol Biol. 1975 Feb 15;92(1):93-111. doi: 10.1016/0022-2836(75)90093-5.
5
Nucleotide sequence of the 5' end of tryptophan messenger RNA of Escherichia coli.大肠杆菌色氨酸信使核糖核酸5′端的核苷酸序列。
J Mol Biol. 1976 May 15;103(2):351-81. doi: 10.1016/0022-2836(76)90317-x.
6
Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli.大肠杆菌色氨酸操纵子前导区的体内转录终止
J Mol Biol. 1976 May 15;103(2):319-37. doi: 10.1016/0022-2836(76)90315-6.
7
Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor.色氨酸操纵子的双重调控由色氨酰 - tRNA合成酶和阻遏物共同介导。
J Mol Biol. 1976 May 15;103(2):209-26. doi: 10.1016/0022-2836(76)90310-7.
8
leu operon of Salmonella typhimurium is controlled by an attenuation mechanism.鼠伤寒沙门氏菌的亮氨酸操纵子受衰减机制调控。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4941-5. doi: 10.1073/pnas.76.10.4941.
9
Single base-pair alterations in the Escherichia coli trp operon leader region that relieve transcription termination at the trp attenuator.大肠杆菌色氨酸操纵子前导区中的单碱基对改变可解除色氨酸衰减子处的转录终止。
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4833-7. doi: 10.1073/pnas.75.10.4833.
10
Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon.大肠杆菌色氨酸操纵子弱化子处转录终止的翻译调控
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5988-92. doi: 10.1073/pnas.75.12.5988.