Elliott T S, Ward J B, Wyrick P B, Rogers H J
J Bacteriol. 1975 Nov;124(2):905-17. doi: 10.1128/jb.124.2.905-917.1975.
The reversion of protoplasts of Bacillus licheniformis 6346 His- on a medium containing 2.5% agar has been studied in sectioned material after reaction with a ferritin-conjugated antibody specific to the peptidoglycan isolated from the walls of the bacilli. Freeze etching has also been used. Fibrils of material reacting with the antibody have been detected emerging from isolated areas of the protoplasts after 3 h of incubation. This material gradually covers the cell and can eventually (at 6 h) be seen in freeze-etched preparations as a fringe of up to 400 nm around the cells and covering the surfaces with particles that can be removed by lysozyme. At later stages the wall begins to take on a compact, well-defined appearance that can be seen in sections; however, the cells are still grossly deformed. A transitory emergence, beyond the wall of long fibers of 6 nm in diameter, takes place after about 12 h of incubation. These fibers react with the conjugated antibody and after freeze etching show a regular banded structure. They are probably indentical with the fibers isolated elsewhere (Elliott et al., 1975) and shown to contain all the wall constituents (i.e., peptidoglycan, teichoic acid, and teichuronic acid). These fibers are not detectable in the final stages of reversion.
用从地衣芽孢杆菌6346 His-细胞壁分离出的肽聚糖特异性铁蛋白偶联抗体进行反应后，已在切片材料中研究了该菌株原生质体在含2.5%琼脂的培养基上的回复情况。还使用了冷冻蚀刻技术。孵育3小时后，已检测到与抗体反应的物质纤维从原生质体的孤立区域出现。这种物质逐渐覆盖细胞，最终（6小时时）在冷冻蚀刻制剂中可见其围绕细胞形成高达400纳米的边缘，并覆盖有可被溶菌酶去除的颗粒的表面。在后期，细胞壁开始呈现出紧密、清晰的外观，这在切片中可见；然而，细胞仍严重变形。孵育约12小时后，直径6纳米的长纤维会短暂地出现在细胞壁之外。这些纤维与偶联抗体反应，冷冻蚀刻后显示出规则的带状结构。它们可能与在其他地方分离出的纤维相同（埃利奥特等人，1975年），并已证明含有所有细胞壁成分（即肽聚糖、磷壁酸和磷壁醛酸）。在回复的最后阶段检测不到这些纤维。
