Arkles B, Brinigar W S
J Biol Chem. 1975 Nov 25;250(22):8856-62.
Rat liver mitochondria are shown to adhere to the alkylsilylated glass beads in essentially a monolayer. The amount of mitochondria bound to the beads reaches a maximum where the length of the alkyl groups covalently linked to the beads exceeds eight carbons. Mitochondria immobilized on the beads and placed in a flow system exhibit normal: (a) respiratory control, (b) phosphate to oxygen ratio, (c) uncoupling by 2,4-dinitrophenol and carbonylcyanide p-trifluoromethoxyphenylhydrazone, and (d) inhibition by cyanide, azide, rotenone, oligomycin, and antimycin. Reversibility of the effects of 2,4-dinitrophenol, cyanide, and azide was rapid and complete. Inhibition by rotenone, oligomycin was essentially irreversible. Mitochondria have been maintained in a viable state on the beads at 27 degrees for periods up to 4 hours. The use of immobilized organelles appears to offer a new technique for the study of membrane-bound particles whereby substances can be rapidly added and removed while monitoring the composition of solution flowing over the particles.
已表明大鼠肝脏线粒体基本上以单层形式附着于烷基硅烷化玻璃珠上。与玻璃珠结合的线粒体数量在与玻璃珠共价连接的烷基长度超过八个碳原子时达到最大值。固定在玻璃珠上并置于流动系统中的线粒体表现出正常的：（a）呼吸控制，（b）磷氧比，（c）被2,4 - 二硝基苯酚和羰基氰对三氟甲氧基苯腙解偶联，以及（d）被氰化物、叠氮化物、鱼藤酮、寡霉素和抗霉素抑制。2,4 - 二硝基苯酚、氰化物和叠氮化物作用的可逆性迅速且完全。鱼藤酮、寡霉素的抑制作用基本上是不可逆的。线粒体在玻璃珠上于27摄氏度下可存活长达4小时。固定化细胞器的使用似乎为研究膜结合颗粒提供了一种新技术，借此在监测流过颗粒的溶液组成时可快速添加和去除物质。
