大鼠肝线粒体中寡霉素对氯离子依赖的偶联恢复作用
Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria.
作者信息
Ariel N, Avi-Dor Y
出版信息
Biochem J. 1975 Oct;152(1):115-9. doi: 10.1042/bj1520115.
In rat liver mitochondria suspended in KC1 medium, oligomycin interfered with the effect of uncouplers on energy conservation. It antagonized the effect of uncouplers that are weak acids (2,4-dinitrophenol etc.), but enhanced that of the lipid-penetrating cation NN-dimethyl-N'N'-dibenzylammonium. Oligomycin caused none of the above effects when Br- or NO-/3 was substituted for C1- as the major anionic species in the assay medium. The concentration of oligomycin that exerted the above-mentioned effects was lower than that necessary for the inhibition of energy transfer, but was in the range that induced C1- permeation through the cristae membrane. The possible connexion between the effect of oligomycin on C1- permeation and its interference with the action of uncouplers is discussed.
在悬浮于氯化钾培养基中的大鼠肝脏线粒体中，寡霉素干扰了解偶联剂对能量保存的作用。它拮抗了弱酸类解偶联剂（2,4-二硝基苯酚等）的作用，但增强了脂溶性阳离子N,N-二甲基-N',N'-二苄基铵的作用。当用溴离子或硝酸根离子取代氯离子作为测定培养基中的主要阴离子时，寡霉素不会产生上述任何一种作用。产生上述作用的寡霉素浓度低于抑制能量转移所需的浓度，但处于诱导氯离子透过嵴膜的范围内。本文讨论了寡霉素对氯离子渗透的作用与其干扰解偶联剂作用之间可能的联系。
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