人脑脊液中5-羟色醇和5-甲氧基色醇的质量碎片分析法

Mass fragmentography of 5-hydroxytryptophol and 5-methoxytryptophol in human cerebrospinal fluid.

作者信息

Curtius H C, Wolfensberger M, Redweik U, Leimbacher W, Maibach R A, Isler W

出版信息

J Chromatogr. 1975 Oct 29;112:523-31. doi: 10.1016/s0021-9673(00)99981-3.

Abstract

A specific and very sensitive method for the determination of 5-hydroxy-tryptophol (5-HTOL) and 5-methoxytryptophol (5-MTOL) in extracts from human cerebrospinal fluid (CSF) involving the use of mass fragmentography and pentafluoropriopionyl derivatives is described. 5-HTOL and 5-MTOL were determined in human CSF of three patients with leukaemia and from nine patients with neurological disorders. The concentration of free 5-HOT in CSF was in the range of 0.1-33 ng/ml and that of 5-MTOL was 0.3-13.9 ng/ml. For the first time the presence of these compounds in human material has been shown. The concentration of these two alcohols in CSF is markedly lower than the concentration of 5-hydroxyindoleacetic acid. These results suggest that human cerebral 5-hydroxytryptamine is preferentially metabolized to 5-HTOL-hydroxyindoleacetic acid rather than to 5-HTOL and 5-MTOL.

摘要

本文描述了一种利用质量碎片分析法和五氟丙酰衍生物测定人脑脊液（CSF）提取物中5-羟色醇（5-HTOL）和5-甲氧基色醇（5-MTOL）的特异性高且灵敏度极高的方法。对3例白血病患者和9例神经系统疾病患者的脑脊液进行了5-HTOL和5-MTOL的测定。脑脊液中游离5-HOT的浓度范围为0.1 - 33 ng/ml，5-MTOL的浓度范围为0.3 - 13.9 ng/ml。首次证实了这些化合物在人体样本中的存在。这两种醇类在脑脊液中的浓度明显低于5-羟吲哚乙酸的浓度。这些结果表明，人脑中的5-羟色胺优先代谢为5-HTOL-羟吲哚乙酸，而非5-HTOL和5-MTOL。