微粒化学键合阴离子交换树脂在核苷酸分析中的性能

The performance of microparticle chemically-bonded anion-exchange resins in the analysis of nucleotides.

作者信息

Hartwick R A, Brown P R

出版信息

J Chromatogr. 1975 Oct 29;112:650-62. doi: 10.1016/s0021-9673(00)99994-1.

Abstract

The performance of microparticles in the ion-exchange chromatographic separation of nucleotides was investigated. A microparticle chemically-bonded strong anion-exchange column packing was used for the analysis of the 5'-mono-, di- and -triphosphate nucleotides of adenine, guanine, hypoxanthine, xanthine, cytosine, uracil and thymine. It was found that excellent resolution of the majority of the nucleotides was obtained at ambient temperatures. The packings were stable and the sample capacity was greater than with pellicular resins. The retention times, peak shapes, heights and areas were highly reproducible and there was good linearity of response. Use of this column packing in biochemical studies is reported.

摘要

研究了微粒在核苷酸离子交换色谱分离中的性能。使用一种化学键合强阴离子交换柱填料微粒，对腺嘌呤、鸟嘌呤、次黄嘌呤、黄嘌呤、胞嘧啶、尿嘧啶和胸腺嘧啶的5'-单磷酸、二磷酸和三磷酸核苷酸进行分析。结果发现，在室温下可获得大多数核苷酸的优异分离度。该填料稳定，样品容量大于薄壳型树脂。保留时间、峰形、峰高和峰面积具有高度重现性，响应呈良好的线性关系。报道了该柱填料在生化研究中的应用。