用于分离单核苷酸的汞化葡聚糖柱色谱法。
Mercurated dextran column chromatography for fractionating mononucleotides.
作者信息
Gruenwedel D W, Fu J C
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2002-5. doi: 10.1073/pnas.68.9.2002.
Abstract
Mercurated dextran (polysaccharide) was found to fractionate mononucleotides according to their affinity for organomercurial Hg(+). The nucleotides are released from the chromatographic column in the sequence CMP, AMP, GMP, and TMP when borate buffer of increasing pH is used as an eluent.
摘要
发现汞化葡聚糖（多糖）可根据单核苷酸对有机汞Hg(+)的亲和力对其进行分离。当使用pH值递增的硼酸盐缓冲液作为洗脱剂时，核苷酸按照CMP、AMP、GMP和TMP的顺序从色谱柱中洗脱出来。
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本文引用的文献
1
Complexing and denaturation of DNA by methylmercuric hydroxide. II. Ultracentrifugation studies.
Biopolymers. 1967;5(9):847-61. doi: 10.1002/bip.1967.360050907.
2
Binding of thiols by a water-insoluble organomercurial copolymer of ethylene and maleic acid.
Arch Biochem Biophys. 1967 Jul;121(1):67-72. doi: 10.1016/0003-9861(67)90010-0.
3
Complexing and denaturation of DNA by methylmercuric hydroxide. I. Spectrophotometric studies.甲基氢氧化汞对DNA的络合与变性。I. 分光光度法研究。
J Mol Biol. 1966 Oct 28;21(1):129-44. doi: 10.1016/0022-2836(66)90084-2.
4
Changes in the sedimentation characteristics of DNA due to methylmercuration.甲基汞化作用导致DNA沉降特性的变化。
Biochem Biophys Res Commun. 1970 Aug 11;40(3):542-8. doi: 10.1016/0006-291x(70)90936-8.
5
A cellulose mercurial: preparation, properties and use in antibody purification.一种纤维素汞制剂：制备、性质及在抗体纯化中的应用。
J Immunol. 1968 Jan;100(1):187-93.
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