免疫抑制对雏火鸡抵抗火鸡冠状病毒感染的保护性免疫的影响。
The effect of immunosuppression on protective immunity of turkey poults against infection with turkey coronavirus.
作者信息
Loa Chien Chang, Lin Tsang Long, Wu Ching Ching, Bryan Thomas, Hooper Tom, Schrader Donna
机构信息
Department of Veterinary Pathobiology, Purdue University, West Lafayette, IN 47907-1175, USA.
出版信息
Comp Immunol Microbiol Infect Dis. 2002 Mar;25(2):127-38. doi: 10.1016/s0147-9571(01)00033-9.
The objective of the present study was to evaluate the protective effect of humoral and cellular immunities on turkeys infected with turkey coronavirus (TCV). Two trials were conducted with two separate hatches of turkey poults. Turkey's were experimentally immunosuppressed with cyclosporin A (CsA) or cyclophosphamide (CY) and infected with TCV. Prior to infection, treatment with CsA selectively suppressed T cell activity as revealed by 2-3 fold decreased (p < 0.1) lymphocyte proliferation responses to a T cell mitogen, concanavalin A (Con A). Treatment with CY mainly induced B cell deficiency as indicated by significant reductions (p < 0.05) in antibody responses to sheep erythrocytes 7 days after injection. Body weight gain of turkeys treated with CY was significantly lower (p < 0.05) than that of untreated turkeys at 9 days post-infection (PI). Turkeys treated with CY had 1-2 fold higher immunofluorescent antibody assay (IFA) scores for TCV antigens (p < 0.05) in the intestine than untreated turkeys at 9 or 14 days PI. These results suggested that humoral immunity against TCV infection may be important in turkeys.
本研究的目的是评估体液免疫和细胞免疫对感染火鸡冠状病毒（TCV）的火鸡的保护作用。用两批不同孵化的火鸡幼雏进行了两项试验。用火鸡通过环孢菌素A（CsA）或环磷酰胺（CY）进行实验性免疫抑制，并感染TCV。在感染前，用CsA处理选择性地抑制了T细胞活性，这表现为对T细胞有丝分裂原刀豆蛋白A（Con A）的淋巴细胞增殖反应降低了2-3倍（p < 0.1）。用CY处理主要诱导B细胞缺陷，表现为注射后7天对绵羊红细胞的抗体反应显著降低（p < 0.05）。在感染后9天（PI），用CY处理的火鸡的体重增加显著低于未处理的火鸡（p < 0.05）。在感染后9天或14天，用CY处理的火鸡肠道中TCV抗原的免疫荧光抗体测定（IFA）评分比未处理的火鸡高1-2倍（p < 0.05）。这些结果表明，针对TCV感染的体液免疫在火鸡中可能很重要。
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