使用酶联免疫吸附测定法对出血性肠炎病毒抗原和抗体进行定量分析。
Quantitation of hemorrhagic enteritis virus antigen and antibody using enzyme-linked immunosorbent assays.
作者信息
van den Hurk J V
出版信息
Avian Dis. 1986 Oct-Dec;30(4):662-71.
Enzyme-linked immunosorbent assays (ELISAs) were developed to quantitate hemorrhagic enteritis virus (HEV) antibodies in turkey sera and HEV antigens in tissue extracts. These assays were more sensitive than the commonly used agar-gel precipitin tests in detecting antigen and antibody. The antibody-ELISA was used to monitor the presence and decline of passive antibodies in turkey poults and the seroconversion of turkeys infected with HEV. The antigen-ELISA was carried out using a monoclonal antibody; this test was used to quantitate HEV antigen in experimentally infected turkeys in a time-sequence experiment. Both ELISAs measured a strong antigenic relationship between an avirulent strain (HEV-A) and a virulent strain (HEV-V).
酶联免疫吸附测定（ELISA）用于定量火鸡血清中的出血性肠炎病毒（HEV）抗体以及组织提取物中的HEV抗原。这些测定在检测抗原和抗体方面比常用的琼脂凝胶沉淀试验更灵敏。抗体ELISA用于监测小火鸡中被动抗体的存在和下降情况以及感染HEV的火鸡的血清转化。抗原ELISA使用单克隆抗体进行；该试验用于在时间序列实验中定量实验感染火鸡中的HEV抗原。两种ELISA都检测到无毒株（HEV-A）和有毒株（HEV-V）之间存在很强的抗原关系。
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