Berfield Anne K, Abrass Christine K
Veteran Affairs Puget Sound Health Care System, Seattle, Washington 98108, USA.
J Histochem Cytochem. 2002 Mar;50(3):395-403. doi: 10.1177/002215540205000310.
When rat glomerular mesangial cells (MCs) are cultured with IGF-1 they accumulate intracellular lipid and take on foam cell morphology. These changes were characterized by electron microscopy and Nile red staining. To define the mechanism responsible for IGF-1-mediated lipid uptake, MCs were evaluated for endocytosis, scavenger receptor activity, and receptor-mediated uptake by the LDL receptor. Lipid accumulation was markedly increased when MCs were cultured with IGF. The primary route of uptake was through enhanced endocytosis. Lipid-laden MCs have decreased phagocytic capacity and disrupted cytoskeletons. These data show that IGF-1 induces MC to take on a foam cell morphology and that lipid-laden MCs have impaired phagocytic function.
当大鼠肾小球系膜细胞（MCs）与胰岛素样生长因子-1（IGF-1）一起培养时，它们会在细胞内积累脂质并呈现出泡沫细胞形态。这些变化通过电子显微镜和尼罗红染色进行了表征。为了确定负责IGF-1介导的脂质摄取的机制，对MCs进行了内吞作用、清道夫受体活性以及低密度脂蛋白（LDL）受体介导的摄取评估。当MCs与IGF一起培养时，脂质积累显著增加。主要的摄取途径是通过增强内吞作用。富含脂质的MCs吞噬能力下降且细胞骨架遭到破坏。这些数据表明，IGF-1诱导MCs呈现出泡沫细胞形态，并且富含脂质的MCs吞噬功能受损。
