Chromosome studies of normal and psoriatic skin fibroblasts.

A method for obtaining large numbers of fibroblasts from punch biopsies of skin tissue and a procedure for the visualization of chromosomes from these cells are presented in detail. The chromosomes are well separated, and fine details of their structure are seen. A study was made of 275 cells from apparently normal and from involved areas of skin from patients with psoriasis. No changes either in the number or in the fine structure of the chromosomes were observed.